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Literature summary extracted from

  • Bengis-Garber, C.; Kushner, D.J.
    Purification and properties of 5'-nucleotidase from the membrane of Vibrio costicola, a moderately halophilic bacterium (1981), J. Bacteriol., 146, 24-32.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane
-
 Salinivibrio costicola 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 KCl activation Salinivibrio costicola
3.1.3.5 Mg2+ activation Salinivibrio costicola
3.1.3.5 Mn2+ activation Salinivibrio costicola
3.1.3.5 NaCl activation Salinivibrio costicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 70000
-
 x * 70000, SDS-PAGE Salinivibrio costicola

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Salinivibrio costicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Salinivibrio costicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 6
-
-
 Salinivibrio costicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O 64% of the activity of ATP Salinivibrio costicola adenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 67% of the activity of ATP Salinivibrio costicola guanosine + phosphate
-
 ?
3.1.3.5 5'-TMP + H2O 107% of the activity of ATP Salinivibrio costicola thymidine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 83% of the activity of ATP Salinivibrio costicola uridine + phosphate
-
 ?
3.1.3.5 ADP + 2 H2O 114% of the activity of ATP Salinivibrio costicola adenosine + 2 phosphate
-
 ?
3.1.3.5 ADP + H2O
-
 Salinivibrio costicola adenosine + diphosphate
-
 ?
3.1.3.5 ATP + 3 H2O
-
 Salinivibrio costicola adenosine + 3 phosphate
-
 ?
3.1.3.5 CTP + H2O 30% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 dATP + H2O 83% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 GDP + H2O 135% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 GTP + H2O 100% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 ITP + H2O 108% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 TTP + H2O 133% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 UDP + H2O 150% of the activity with ATP Salinivibrio costicola ?
-
 ?
3.1.3.5 UTP + H2O 53% of the activity with ATP Salinivibrio costicola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 70000, SDS-PAGE Salinivibrio costicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 8.2
-
-
 Salinivibrio costicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.5 7 9 less than 50% of maximal activity above and below Salinivibrio costicola