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Literature summary extracted from

  • Naito, Y.; Lowenstein, J.M.
    5'-Nucleotidase from rat heart (1981), Biochemistry, 20, 5188-5194.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.002
-
 adenosine 5'-O-thiophosphate
-
 Rattus norvegicus
3.1.3.5 0.0053
-
 6-chloropurine ribonucleoside 5'-monophosphate
-
 Rattus norvegicus
3.1.3.5 0.0056
-
 5'-AMP
-
 Rattus norvegicus
3.1.3.5 0.0064
-
 5'-GMP
-
 Rattus norvegicus
3.1.3.5 0.0065
-
 1,N6-ethenoadenosine 5'-phosphate
-
 Rattus norvegicus
3.1.3.5 0.007
-
 3-beta-ribofuranosyladenine 5'-monophosphate
-
 Rattus norvegicus
3.1.3.5 0.008
-
 5'-IMP
-
 Rattus norvegicus
3.1.3.5 0.014
-
 5'-UMP
-
 Rattus norvegicus
3.1.3.5 0.014
-
 5'-dTMP
-
 Rattus norvegicus
3.1.3.5 0.035
-
 5'-CMP
-
 Rattus norvegicus
3.1.3.5 0.077
-
 5'-dAMP
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane
-
 Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ activation, membrane-bound enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 74000
-
 2 * 74000, heart, membrane-bound, SDS-PAGE Rattus norvegicus
3.1.3.5 147000
-
 heart, membrane-bound, SDS-PAGE after cross-linkage with dimethylsuberimidate Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 side-chain modification glycoprotein Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 heart, membrane-bound Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 heart
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 4°C, 50 mM Tris-HCl, pH 7.5, 50 mM sodium cholate, 1 M NaCl Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 1,N6-ethenoadenosine 5'-phosphate + H2O 45% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus 1,N6-ethenoadenosine ribonucleoside + phosphate
-
 ?
3.1.3.5 3-beta-ribofuranosyladenine 5'-monophosphate + H2O 24% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus 3-beta-ribofuranosyladenine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O
-
 Rattus norvegicus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 87% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus cytidine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Rattus norvegicus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O 20% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O 28% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dCMP + H2O i.e. 2'-deoxycytosine 5'-phosphate Rattus norvegicus deoxycytidine + phosphate
-
 ?
3.1.3.5 5'-dTMP + H2O i.e. 2'-deoxythymidine 5'-phosphate Rattus norvegicus deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-dTMP + H2O 55% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus deoxythymidine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 50% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O 55% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 93% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus uridine + phosphate
-
 ?
3.1.3.5 6-chloropurine ribonucleoside 5'-monophosphate + H2O 50% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus 6-chloropurine ribonucleoside + phosphate
-
 ?
3.1.3.5 adenosine 5'-O-thiophosphate + H2O 6% of the activity with 5'-AMP, heart, membrane-bound enzyme Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 dimer 2 * 74000, heart, membrane-bound, SDS-PAGE Rattus norvegicus