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Literature summary extracted from

  • Camici, M.; Fini, C.; Ipata, P.L.
    Isolation and kinetic properties of 5'-nucleotidase from guinea-pig skeletal muscle (1985), Biochim. Biophys. Acta, 840, 6-12.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 ADP
-
 Cavia porcellus
3.1.3.5 ATP
-
 Cavia porcellus
3.1.3.5 Ca2+
-
 Cavia porcellus
3.1.3.5 concanavalin A
-
 Cavia porcellus
3.1.3.5 EDTA
-
 Cavia porcellus
3.1.3.5 Mg2+
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.019
-
 5'-AMP
-
 Cavia porcellus
3.1.3.5 0.05
-
 5'-dAMP
-
 Cavia porcellus
3.1.3.5 0.059
-
 5'-GMP
-
 Cavia porcellus
3.1.3.5 0.1
-
 5'-IMP
-
 Cavia porcellus
3.1.3.5 0.11
-
 5'-UMP
-
 Cavia porcellus
3.1.3.5 0.36
-
 5'-CMP
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane
-
 Cavia porcellus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mn2+ activation Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 71000
-
 x * 71000, SDS-PAGE Cavia porcellus
3.1.3.5 185000
-
 gel filtration Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 skeletal muscle
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 40.4
-
-
 Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -20°C Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Cavia porcellus adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 125% of the activity with 5'-AMP Cavia porcellus cytidine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Cavia porcellus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O 22% of the activity with 5'-AMP Cavia porcellus deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 117% of the activity with 5'-AMP Cavia porcellus guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O 92% of the activity with 5'-AMP Cavia porcellus inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 117% of the activity with 5'-AMP Cavia porcellus uridine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 71000, SDS-PAGE Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.5 45
-
 15 min stable Cavia porcellus
3.1.3.5 56
-
 15 min, 20% loss of activity Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 8 9
-
 Cavia porcellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.5 5 9
-
 Cavia porcellus