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Literature summary extracted from

  • Carter, S.G.; Tipton, C.L.
    Purification and characterization of a 5'-nucleotidase from Zea mays microsomes (1986), Phytochemistry, 25, 33-37.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 6-Chloropurine riboside
-
Zea mays
3.1.3.5 6-gamma,gamma-dimethylallylaminopurine riboside
-
Zea mays
3.1.3.5 6-mercaptopurine riboside
-
Zea mays
3.1.3.5 adenosine
-
Zea mays
3.1.3.5 adenosine 5'-sulfate
-
Zea mays
3.1.3.5 ATP
-
Zea mays
3.1.3.5 cAMP
-
Zea mays
3.1.3.5 kinstin riboside
-
Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.0571
-
5'-GMP
-
Zea mays
3.1.3.5 0.0577
-
5'-AMP
-
Zea mays
3.1.3.5 0.0816
-
5'-IMP
-
Zea mays
3.1.3.5 0.2
-
5'-UMP
-
Zea mays
3.1.3.5 0.3333
-
5'-CMP
-
Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 microsome
-
Zea mays
-
-
3.1.3.5 plasma membrane
-
Zea mays 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 24500
-
1 * 24500 + 1 * 25500, SDS-PAGE Zea mays
3.1.3.5 25500
-
1 * 24500 + 1 * 25500, SDS-PAGE Zea mays
3.1.3.5 49000
-
gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Zea mays
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 side-chain modification 40% carbohydrate Zea mays

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 seedling
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 0.73
-
-
Zea mays

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -20°C, several months Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
Zea mays adenosine + phosphate
-
?
3.1.3.5 5'-CMP + H2O 116% of the activity with 5'-AMP Zea mays cytidine + phosphate
-
?
3.1.3.5 5'-CMP + H2O 46% of the activity with 5'-AMP Zea mays cytidine + phosphate
-
?
3.1.3.5 5'-dIMP + H2O
-
Zea mays deoxyinosine + phosphate
-
?
3.1.3.5 5'-GMP + H2O 116% of the activity with 5'-AMP Zea mays guanosine + phosphate
-
?
3.1.3.5 5'-UMP + H2O 71% of the activity with 5'-AMP Zea mays uridine + phosphate
-
?
3.1.3.5 5'-UMP + H2O 137% of the activity with 5'-AMP Zea mays uridine + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 dimer 1 * 24500 + 1 * 25500, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 additional information
-
no clear optimum Zea mays