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Literature summary extracted from

  • Grondal, E.J.M.; Zimmermann, H.
    Purification, characterization and cellular localization of 5'-nucleotidase from Torpedo electric organ (1987), Biochem. J., 245, 805-810.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 adenosine 5'-(alpha,beta-methylene)diphosphate
-
 Torpedo marmorata
3.1.3.5 concanavalin A
-
 Torpedo marmorata
3.1.3.5 Pb2+
-
 Torpedo marmorata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.5 0.038
-
 5'-AMP
-
 Torpedo marmorata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane
-
 Torpedo marmorata 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mg2+ slight activation Torpedo marmorata
3.1.3.5 Sr2+ slight activation Torpedo marmorata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 62000
-
 2 * 62000, SDS-PAGE Torpedo marmorata
3.1.3.5 131000
-
 gel filtration Torpedo marmorata

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Torpedo marmorata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.5 side-chain modification glycoprotein Torpedo marmorata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Torpedo marmorata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 electric organ enriched in nerve-endings Torpedo marmorata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 31.26
-
-
 Torpedo marmorata

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -18°C Torpedo marmorata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Torpedo marmorata adenosine + phosphate
-
 ?
3.1.3.5 5'-CMP + H2O 93% of the activity with 5'-AMP Torpedo marmorata cytidine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O i.e. 2'-deoxyadenosine 5'-phosphate Torpedo marmorata deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-dAMP + H2O 14% of the activity with 5'-AMP Torpedo marmorata deoxyadenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O 52% of the activity with 5'-AMP Torpedo marmorata guanosine + phosphate
-
 ?
3.1.3.5 5'-IMP + H2O 63% of the activity with 5'-AMP Torpedo marmorata inosine + phosphate
-
 ?
3.1.3.5 5'-UMP + H2O 80% of the activity with 5'-AMP Torpedo marmorata uridine + phosphate
-
 ?
3.1.3.5 ADP + H2O 4.5% of the activity with 5'-AMP Torpedo marmorata adenosine + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 dimer 2 * 62000, SDS-PAGE Torpedo marmorata