Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Itami, H.; Sakai, Y.; Shimamoto, T.; Hama, H.; Tsuda, M.; Tsuciya, T.
    Purification and characterization of membrane-bound 5'-nucleotidase of Vibrio parahaemolyticus (1989), J. Biochem., 105, 785-789.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.5 azolectin stimulation Vibrio parahaemolyticus

General Stability

EC Number General Stability Organism
3.1.3.5 inactivation by repeated freezing and thawing Vibrio parahaemolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 Ni2+
-
 Vibrio parahaemolyticus
3.1.3.5 Zn2+
-
 Vibrio parahaemolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.5 plasma membrane the catalytic site is oriented outward Vibrio parahaemolyticus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Br- can substitute for Cl- in activation Vibrio parahaemolyticus
3.1.3.5 Cl- 20 mM optimal for activity Vibrio parahaemolyticus
3.1.3.5 Co2+ 40% as effective as Mg2+ Vibrio parahaemolyticus
3.1.3.5 Mg2+ dependent on Vibrio parahaemolyticus
3.1.3.5 Mn2+ 40% as effective as Mg2+ Vibrio parahaemolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 72000
-
 x * 72000, SDS-PAGE Vibrio parahaemolyticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Vibrio parahaemolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5
-
 Vibrio parahaemolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.5 1400
-
-
 Vibrio parahaemolyticus

Storage Stability

EC Number Storage Stability Organism
3.1.3.5 -80°C, purified enzyme, stable for at least 6 months Vibrio parahaemolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Vibrio parahaemolyticus adenosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 ? x * 72000, SDS-PAGE Vibrio parahaemolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.5 8
-
-
 Vibrio parahaemolyticus