BRENDA - Enzyme Database

Inactivation of rat liver HMG-CoA reductase phosphatases by polycarboxylic acids

Hegardt G.; Gil G.; Calvet E.; J. Lipid Res. 24, 821-830 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.47
3-hydroxy-3-methylglutarate
-
Rattus norvegicus
3.1.3.47
citrate
-
Rattus norvegicus
3.1.3.47
diphosphate
-
Rattus norvegicus
3.1.3.47
Maleate
-
Rattus norvegicus
3.1.3.47
malonate
-
Rattus norvegicus
3.1.3.47
oxalate
-
Rattus norvegicus
3.1.3.47
phosphate
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.47
Mg2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
3.1.3.47
Mn2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.47
310000
-
cytosolic isoenzyme IIa, gel chromatography
Rattus norvegicus
3.1.3.47
480000
-
cytosolic isoenzyme Ia, gel chromatography
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
Rattus norvegicus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.47
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.47
liver
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.47
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.47
310
315
no comments on grade of purification, native cytosolic isoenzyme Ia
Rattus norvegicus
3.1.3.47
670
710
no comments on grade of purification, native cytosolic isoenzyme IIa
Rattus norvegicus
3.1.3.47
21480
-
purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.47
glycogen synthase phosphate + H2O
-
94957
Rattus norvegicus
glycogen synthase + phosphate
-
94957
Rattus norvegicus
?
3.1.3.47
phosphorylase a phosphate + H2O
-
94957
Rattus norvegicus
phosphorylase a + phosphate
-
94957
Rattus norvegicus
?
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
94957
Rattus norvegicus
?
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
?
-
-
-
-
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.47
3-hydroxy-3-methylglutarate
-
Rattus norvegicus
3.1.3.47
citrate
-
Rattus norvegicus
3.1.3.47
diphosphate
-
Rattus norvegicus
3.1.3.47
Maleate
-
Rattus norvegicus
3.1.3.47
malonate
-
Rattus norvegicus
3.1.3.47
oxalate
-
Rattus norvegicus
3.1.3.47
phosphate
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.47
Mg2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
3.1.3.47
Mn2+
activation, inactivation by chelators of divalent cations, metal bound at the catalytic site
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.47
310000
-
cytosolic isoenzyme IIa, gel chromatography
Rattus norvegicus
3.1.3.47
480000
-
cytosolic isoenzyme Ia, gel chromatography
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
Rattus norvegicus
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.47
liver
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.47
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.47
310
315
no comments on grade of purification, native cytosolic isoenzyme Ia
Rattus norvegicus
3.1.3.47
670
710
no comments on grade of purification, native cytosolic isoenzyme IIa
Rattus norvegicus
3.1.3.47
21480
-
purification 4500fold, catalytic subunit of ~35000 Da after ethanol treatment
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.47
glycogen synthase phosphate + H2O
-
94957
Rattus norvegicus
glycogen synthase + phosphate
-
94957
Rattus norvegicus
?
3.1.3.47
phosphorylase a phosphate + H2O
-
94957
Rattus norvegicus
phosphorylase a + phosphate
-
94957
Rattus norvegicus
?
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
[hydroxymethylglutaryl-CoA reductase (NADPH)] + phosphate
-
94957
Rattus norvegicus
?
3.1.3.47
[hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate + H2O
-
94957
Rattus norvegicus
?
-
-
-
-