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Literature summary extracted from

  • Pilkis, S.J.; Claus, T.H.; Kountz, P.D.; El-Maghrabi, M.R.
    Enzymes of the fructose 6-phosphate-fructose 1,6-bisphosphate substrate cycle (1987), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 18, 3-46.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.46 ATP
-
 Rattus norvegicus
3.1.3.46 glycerol 1-phosphate activation at high substrate concentrations Rattus norvegicus
3.1.3.46 GTP
-
 Rattus norvegicus
3.1.3.46 phosphate activates at high substrate concentrations Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.46 2,5-anhydro-D-mannitol 6-phosphate
-
 Rattus norvegicus
3.1.3.46 D-psicose 6-phosphate poor inhibitor Rattus norvegicus
3.1.3.46 D-ribose 5-phosphate poor inhibitor Rattus norvegicus
3.1.3.46 D-tagatose 6-phosphate poor inhibitor Rattus norvegicus
3.1.3.46 diethyldicarbonate inactivation Rattus norvegicus
3.1.3.46 fructose 6-phosphate noncompetetive Bos taurus
3.1.3.46 fructose 6-phosphate noncompetetive Rattus norvegicus
3.1.3.46 glycerol 1-phosphate inhibition at low substrate concentrations Rattus norvegicus
3.1.3.46 L-Sorbose 6-phosphate
-
 Rattus norvegicus
3.1.3.46 p-mercuribenzoate
-
 Rattus norvegicus
3.1.3.46 phosphate
-
 Bos taurus
3.1.3.46 phosphate inhibition at low substrate concentrations Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.46 0.0001
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus
3.1.3.46 0.02
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.46 cytosol
-
 Rattus norvegicus 5829
-
3.1.3.46 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.46 100000
-
 gel filtration or sucrose density gradient centrifugation Rattus norvegicus
3.1.3.46 100000
-
 gel filtration or sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O Rattus norvegicus
-
 ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O Bos taurus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.46 Bos taurus
-
 overview
-
3.1.3.46 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Rattus norvegicus
a
3.1.3.46 beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate bifunctional enzyme: 6-phosphofructo-2-kinase-fructose-2,6-bisphosphatase Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.46 liver
-
 Rattus norvegicus
-
3.1.3.46 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.46 20 40
-
 Rattus norvegicus
3.1.3.46 20 40
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.1.3.46 -70°C for 3 months including 100 mM KCl, 0.5 mM dithiothreitol, 20% glycerol and 50 mM Tris-HCl at pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Bos taurus fructose 6-phosphate + phosphate
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.3.46 fructose 2,6-bisphosphate + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.46 dimer 2 * 50000-55000, SDS-PAGE Rattus norvegicus
3.1.3.46 dimer 2 * 50000-55000, SDS-PAGE Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.46 0.1
-
 fructose 2,6-bisphosphate
-
 Rattus norvegicus