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Literature summary extracted from
- Expression of the catalytic subunit of phosphorylase phosphatase (protein phosphatase-1) in Escherichia coli (1992), J. Biol. Chem., 267, 1484-1490.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.17 | expression in Escherichia coli. Expression of the enzyme in the pET3a vector results in the expression of the enzyme as an insoluble aggregate. The vector with the trp-lac hybrid promotor the enzyme is expressed as a soluble protein at levels of 3-4% of the soluble Escherichia coli protein | Oryctolagus cuniculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.17 | microcystin-LR | - |
Oryctolagus cuniculus |  |
| 3.1.3.17 | okadaic acid | - |
Oryctolagus cuniculus | |
| 3.1.3.17 | protein | modulator protein, inhibitor 2 | Oryctolagus cuniculus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.17 | Mn2+ | required | Oryctolagus cuniculus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.17 | 37000 | - |
x * 37000, SDS-PAGE | Oryctolagus cuniculus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.17 | Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.17 | recombinant enzyme | Oryctolagus cuniculus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.17 | muscle | skeletal | Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.17 | additional information | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.17 | phosphorylase a + H2O | - |
Oryctolagus cuniculus | phosphorylase b + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.17 | ? | x * 37000, SDS-PAGE | Oryctolagus cuniculus |
| 3.1.3.17 | monomer | - |
Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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