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Literature summary extracted from

  • McNall, S.J.; Ballou, L.M.; Villa-Moruzzi, E.; Fischer, E.H.
    Purification and characterization of phosphorylase phosphatase from rabbit skeletal muscle (1988), Methods Enzymol., 159, 377-390.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.17 additional information the inactive enzyme can be activated in two ways: 1. by limited proteolysis with trypsin in presence of Mn2+, 2. activation by an MgATP2--dependent reaction, that also requires a factor known as Fa, which appears to be identical to glycogen synthase kinase-3 Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
3.1.3.17 the 31000 Da regulatory subunit is extremely susceptible to proteolysis, rapidly destroyed by incubation with trypsin Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.17 protein heat stable proteins: inhibitor 1 and 2 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.17 additional information the inactive enzyme can be activated in two ways: 1. by limited proteolysis with trypsin in presence of Mn2+, 2. activation by an MgATP2--dependent reaction, that also requires a factor known as Fa, which appears to be identical to glycogen synthase kinase-3 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.17 31000
-
 1 * 31000 regulatory subunit + 1 * 38000 catalytic subunit, SDS-PAGE Oryctolagus cuniculus
3.1.3.17 38000
-
 1 * 31000 regulatory subunit + 1 * 38000 catalytic subunit, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.17 additional information Oryctolagus cuniculus the enzyme plays an important role in carbohydrate metabolism by affecting the activity of phosphorylase, glycogen synthase and phosphorylase kinase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.17 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.17
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.17 muscle skeletal Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.17 additional information the enzyme plays an important role in carbohydrate metabolism by affecting the activity of phosphorylase, glycogen synthase and phosphorylase kinase Oryctolagus cuniculus ?
-
 ?
3.1.3.17 phosphorylase a + H2O
-
 Oryctolagus cuniculus phosphorylase b + phosphate
-
 ?
3.1.3.17 phosphorylase kinase + H2O preferentially dephosphorylates the beta subunit over the alpha subunit Oryctolagus cuniculus phosphate + dephosphorylated phosphorylase kinase
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.17 dimer 1 * 31000 regulatory subunit + 1 * 38000 catalytic subunit, SDS-PAGE Oryctolagus cuniculus