BRENDA - Enzyme Database

Properties of amphiphilic and hydrophilic forms of alkaline phosphatase from human liver

Kihn, L.; Rutkowski, D.; Nakatsui, T.; Stinson, R.A.; Enzyme 45, 155-164 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.1
albumin
3fold stimulation of hydrophilic enzyme, slight stimulation of amphiphilic enzyme
Homo sapiens
3.1.3.1
phosphatidylcholine
1fold stimulation of hydrophilic enzyme form
Homo sapiens
3.1.3.1
phosphatidylethanolamine
1fold stimulation of hydrophilic enzyme form
Homo sapiens
3.1.3.1
phosphatidylinositol
1.5fold stimulation of hydrophilic enzyme form
Homo sapiens
3.1.3.1
phosphatidylserine
1fold stimulation of amphiphilic enzyme form, 3fold stimulation of hydrophilic enzyme form
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.1
0.02
-
p-nitrophenyl phosphate
pH 9.0, hydrophilic and amphiphilic enzyme form
Homo sapiens
3.1.3.1
4.48
-
p-nitrophenyl phosphate
pH 10.3, hydrophilic enzyme form
Homo sapiens
3.1.3.1
5.02
-
p-nitrophenyl phosphate
pH 10.3, amphiphilic enzyme form
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.1
membrane
covalent attachment of the hydrophobic phosphatidylinositol membrane anchor causes the amphiphilic form to behave anomalously on electrophoresis
Homo sapiens
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.1
214000
-
hydrophilic enzyme form, gradient gel electrophoresis
Homo sapiens
3.1.3.1
255000
-
hydrophilic enzyme form, gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.1
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.1
liver
amphiphilic and hydrophilic enzyme form
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
p-nitrophenyl phosphate + H2O
-
94623
Homo sapiens
p-nitrophenol + phosphate
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.1
albumin
3fold stimulation of hydrophilic enzyme, slight stimulation of amphiphilic enzyme
Homo sapiens
3.1.3.1
phosphatidylcholine
1fold stimulation of hydrophilic enzyme form
Homo sapiens
3.1.3.1
phosphatidylethanolamine
1fold stimulation of hydrophilic enzyme form
Homo sapiens
3.1.3.1
phosphatidylinositol
1.5fold stimulation of hydrophilic enzyme form
Homo sapiens
3.1.3.1
phosphatidylserine
1fold stimulation of amphiphilic enzyme form, 3fold stimulation of hydrophilic enzyme form
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.1
0.02
-
p-nitrophenyl phosphate
pH 9.0, hydrophilic and amphiphilic enzyme form
Homo sapiens
3.1.3.1
4.48
-
p-nitrophenyl phosphate
pH 10.3, hydrophilic enzyme form
Homo sapiens
3.1.3.1
5.02
-
p-nitrophenyl phosphate
pH 10.3, amphiphilic enzyme form
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.1
membrane
covalent attachment of the hydrophobic phosphatidylinositol membrane anchor causes the amphiphilic form to behave anomalously on electrophoresis
Homo sapiens
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.1
214000
-
hydrophilic enzyme form, gradient gel electrophoresis
Homo sapiens
3.1.3.1
255000
-
hydrophilic enzyme form, gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.1
liver
amphiphilic and hydrophilic enzyme form
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
p-nitrophenyl phosphate + H2O
-
94623
Homo sapiens
p-nitrophenol + phosphate
-
-
-
?