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Literature summary extracted from
- Characterization and comparison of soluble and membranous forms of intestinal alkaline phosphatase from the suckling rat (1981), J. Biol. Chem., 256, 5620-5626.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | Phe | 20 mM, 82-99% inhibition of the soluble enzyme form, 68-95% inhibition of the particulate enzyme form | Rattus norvegicus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.1 | 0.3 | - |
beta-Glycerophosphate | soluble enzyme form | Rattus norvegicus | |
| 3.1.3.1 | 0.38 | - |
beta-Glycerophosphate | membranous enzyme form | Rattus norvegicus | |
| 3.1.3.1 | 0.39 | - |
ATP | membranous enzyme form | Rattus norvegicus | |
| 3.1.3.1 | 0.44 | - |
p-nitrophenyl phosphate | membranous enzyme form | Rattus norvegicus | |
| 3.1.3.1 | 0.45 | - |
p-nitrophenyl phosphate | soluble enzyme form | Rattus norvegicus | |
| 3.1.3.1 | 0.48 | - |
ATP | soluble enzyme form | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.1 | soluble | - |
Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 47000 | - |
2 * 47000, soluble enzyme form, SDS-PAGE | Rattus norvegicus |
| 3.1.3.1 | 56000 | - |
2 * 56000, membranous enzyme form, SDS-PAGE | Rattus norvegicus |
| 3.1.3.1 | 95000 | - |
soluble enzyme form, gel filtration | Rattus norvegicus |
| 3.1.3.1 | 98000 | - |
membranous enzyme form, gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | side-chain modification | glycoprotein, the soluble form contains more fucose and N-acetylgalactosamine than does the membranous form. The soluble enzyme form contains 19.8% carbohydrate, the membranous enzyme form contains 16% carbohydrate | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | intestine | the soluble and the membranous form are closely related but distinct isoenzymes | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 147 | - |
membranous isoenzyme | Rattus norvegicus |
| 3.1.3.1 | 341 | - |
soluble isoenzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.1 | 2-phosphoglycerate + H2O | - |
Rattus norvegicus | glycerol + phosphate | - |
? | |
| 3.1.3.1 | ATP + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.1.3.1 | beta-glycerophosphate + H2O | - |
Rattus norvegicus | glycerol + phosphate | - |
? | |
| 3.1.3.1 | p-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | dimer | 2 * 56000, membranous enzyme form, SDS-PAGE | Rattus norvegicus |
| 3.1.3.1 | dimer | 2 * 47000, soluble enzyme form, SDS-PAGE | Rattus norvegicus |
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