BRENDA - Enzyme Database

Multiple forms and some properties of alkaline phosphatase produced by Aspergillus oryzae on solid medium

Sakurai, Y.; Toda, K.; Shiota, H.; Agric. Biol. Chem. 45, 1959-1967 (1981)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.1
arsenate
1 mM Na2HAsO4, 80% inhibition of isoenzyme I, 22% inhibition of isoenzyme II, 40% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
Ba2+
1 mM, 95% inhibition of isoenzyme I
Aspergillus oryzae
3.1.3.1
Borate
1 mM H3BO4, 16% inhibition of isoenzyme I, 13% inhibition of isoenzyme II, 8% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
Ca2+
weak inhibition of isoenzyme II
Aspergillus oryzae
3.1.3.1
Cu2+
1 mM, 87% inhibition of isoenzyme I, 10% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
EDTA
inhibition of isoenzyme I and II
Aspergillus oryzae
3.1.3.1
F-
1 mM NaF, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II
Aspergillus oryzae
3.1.3.1
Hg2+
1 mM nearly complete inhibition of isoenzyme I, II and III
Aspergillus oryzae
3.1.3.1
Mg2+
weak inhibition of isoenzyme I
Aspergillus oryzae
3.1.3.1
Mn2+
1 mM 90% inhibition of isoenzyme I, 64% inhibition of isoenzyme II, 39% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
NaWO4
1 mM NaWO4, 35% inhibition of isoenzyme I, 80% inhibition of isoenzyme II, 14% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
PCMB
1 mM, 54% inhibition of isoenzyme I, 33% inhibition of isoenzyme II, 9% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
phosphate
1 mM Na2HPO4, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II, 2% inhibition of isoenzyme III; competitive
Aspergillus oryzae
3.1.3.1
Sr2+
1 mM, 88% inhibition of isoenzyme I
Aspergillus oryzae
3.1.3.1
Zn2+
1 mM, 98% inhibition of isoenzyme I
Aspergillus oryzae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.1
0.11
-
p-nitrophenyl phosphate
isoenzyme III
Aspergillus oryzae
3.1.3.1
0.22
-
p-nitrophenyl phosphate
isoenzyme II
Aspergillus oryzae
3.1.3.1
2
-
p-nitrophenyl phosphate
isoenzyme I
Aspergillus oryzae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.1
Ca2+
activates isoenzyme I, weak activation of isoenzyme III
Aspergillus oryzae
3.1.3.1
Mg2+
activation of isoenzyme II and III
Aspergillus oryzae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.1
Aspergillus oryzae
-
three isoenzymes: I, II, III
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
2',3'-CMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
2',3'-GMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
2',3-AMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
2-phosphoglycerate + H2O
hydrolyzed by isoenzyme III, weak activity with isoenzyme I and II
94607
Aspergillus oryzae
glycerol + phosphate
-
-
-
?
3.1.3.1
3-phosphoglycerate + H2O
hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I
94607
Aspergillus oryzae
glycerol + phosphate
-
-
-
?
3.1.3.1
5'-AMP + H2O
-
94607
Aspergillus oryzae
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-dCMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
5'-GMP + H2O
-
94607
Aspergillus oryzae
guanosine + phosphate
-
-
-
?
3.1.3.1
alpha-naphthyl phosphate + H2O
hydrolyzed by isoenzyme II and III, no activity with isoenzyme I
94607
Aspergillus oryzae
naphthol + phosphate
-
-
-
?
3.1.3.1
p-nitrophenyl phosphate + H2O
hydrolyzed by isoenzyme I, II or III
94607
Aspergillus oryzae
p-nitrophenol + phosphate
-
-
-
?
3.1.3.1
phosphoethanolamine + H2O
hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I
94607
Aspergillus oryzae
phosphate + ethanolamine
-
-
-
?
3.1.3.1
phosphorylcholine + H2O
hydrolyzed by isoenzyme III, no activity with isoenzyme I and II
94607
Aspergillus oryzae
phosphate + choline
-
-
-
?
3.1.3.1
phosphoserine + H2O
hydrolyzed by isoenzyme II, weak activity with isoenzyme I and II
94607
Aspergillus oryzae
phosphate + Ser
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.1
30
-
pH 8, 120 min, stable
Aspergillus oryzae
3.1.3.1
35
-
pH 10, isoenzyme III, 120 min, stable
Aspergillus oryzae
3.1.3.1
40
-
pH 9-9.5, 120 min, stable
Aspergillus oryzae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.1
8
-
isoenzyme I
Aspergillus oryzae
3.1.3.1
9
9.5
isoenzyme II
Aspergillus oryzae
3.1.3.1
10
-
isoenzyme III
Aspergillus oryzae
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.3.1
7
10
30°C, stable
Aspergillus oryzae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.1
arsenate
1 mM Na2HAsO4, 80% inhibition of isoenzyme I, 22% inhibition of isoenzyme II, 40% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
Ba2+
1 mM, 95% inhibition of isoenzyme I
Aspergillus oryzae
3.1.3.1
Borate
1 mM H3BO4, 16% inhibition of isoenzyme I, 13% inhibition of isoenzyme II, 8% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
Ca2+
weak inhibition of isoenzyme II
Aspergillus oryzae
3.1.3.1
Cu2+
1 mM, 87% inhibition of isoenzyme I, 10% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
EDTA
inhibition of isoenzyme I and II
Aspergillus oryzae
3.1.3.1
F-
1 mM NaF, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II
Aspergillus oryzae
3.1.3.1
Hg2+
1 mM nearly complete inhibition of isoenzyme I, II and III
Aspergillus oryzae
3.1.3.1
Mg2+
weak inhibition of isoenzyme I
Aspergillus oryzae
3.1.3.1
Mn2+
1 mM 90% inhibition of isoenzyme I, 64% inhibition of isoenzyme II, 39% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
NaWO4
1 mM NaWO4, 35% inhibition of isoenzyme I, 80% inhibition of isoenzyme II, 14% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
PCMB
1 mM, 54% inhibition of isoenzyme I, 33% inhibition of isoenzyme II, 9% inhibition of isoenzyme III
Aspergillus oryzae
3.1.3.1
phosphate
1 mM Na2HPO4, 74% inhibition of isoenzyme I, 15% inhibition of isoenzyme II, 2% inhibition of isoenzyme III; competitive
Aspergillus oryzae
3.1.3.1
Sr2+
1 mM, 88% inhibition of isoenzyme I
Aspergillus oryzae
3.1.3.1
Zn2+
1 mM, 98% inhibition of isoenzyme I
Aspergillus oryzae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.1
0.11
-
p-nitrophenyl phosphate
isoenzyme III
Aspergillus oryzae
3.1.3.1
0.22
-
p-nitrophenyl phosphate
isoenzyme II
Aspergillus oryzae
3.1.3.1
2
-
p-nitrophenyl phosphate
isoenzyme I
Aspergillus oryzae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.1
Ca2+
activates isoenzyme I, weak activation of isoenzyme III
Aspergillus oryzae
3.1.3.1
Mg2+
activation of isoenzyme II and III
Aspergillus oryzae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
2',3'-CMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
2',3'-GMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
2',3-AMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
2-phosphoglycerate + H2O
hydrolyzed by isoenzyme III, weak activity with isoenzyme I and II
94607
Aspergillus oryzae
glycerol + phosphate
-
-
-
?
3.1.3.1
3-phosphoglycerate + H2O
hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I
94607
Aspergillus oryzae
glycerol + phosphate
-
-
-
?
3.1.3.1
5'-AMP + H2O
-
94607
Aspergillus oryzae
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-dCMP + H2O
-
94607
Aspergillus oryzae
?
-
-
-
?
3.1.3.1
5'-GMP + H2O
-
94607
Aspergillus oryzae
guanosine + phosphate
-
-
-
?
3.1.3.1
alpha-naphthyl phosphate + H2O
hydrolyzed by isoenzyme II and III, no activity with isoenzyme I
94607
Aspergillus oryzae
naphthol + phosphate
-
-
-
?
3.1.3.1
p-nitrophenyl phosphate + H2O
hydrolyzed by isoenzyme I, II or III
94607
Aspergillus oryzae
p-nitrophenol + phosphate
-
-
-
?
3.1.3.1
phosphoethanolamine + H2O
hydrolyzed by isoenzyme III, weak activity with isoenzyme II, no activity with isoenzyme I
94607
Aspergillus oryzae
phosphate + ethanolamine
-
-
-
?
3.1.3.1
phosphorylcholine + H2O
hydrolyzed by isoenzyme III, no activity with isoenzyme I and II
94607
Aspergillus oryzae
phosphate + choline
-
-
-
?
3.1.3.1
phosphoserine + H2O
hydrolyzed by isoenzyme II, weak activity with isoenzyme I and II
94607
Aspergillus oryzae
phosphate + Ser
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.1
30
-
pH 8, 120 min, stable
Aspergillus oryzae
3.1.3.1
35
-
pH 10, isoenzyme III, 120 min, stable
Aspergillus oryzae
3.1.3.1
40
-
pH 9-9.5, 120 min, stable
Aspergillus oryzae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.1
8
-
isoenzyme I
Aspergillus oryzae
3.1.3.1
9
9.5
isoenzyme II
Aspergillus oryzae
3.1.3.1
10
-
isoenzyme III
Aspergillus oryzae
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.3.1
7
10
30°C, stable
Aspergillus oryzae