BRENDA - Enzyme Database

Purification and characterization of the outer membrane associated alkaline phosphatase of Lysobacter enzymogenes

von Tigerstrom R.G.; Stelmaschuk, S.; J. Gen. Microbiol. 132, 1379-1387 (1986)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.1
8-hydroxyquinoline
-
Lysobacter enzymogenes
3.1.3.1
EDTA
-
Lysobacter enzymogenes
3.1.3.1
phosphate
insensitive
Lysobacter enzymogenes
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.1
0.034
-
p-nitrophenyl phosphate
and a second Km value of 0.056 mM
Lysobacter enzymogenes
3.1.3.1
0.056
-
p-nitrophenyl phosphate
and a second Km value of 0.034 mM
Lysobacter enzymogenes
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.1
membrane
enzyme is associated with the outer membrane
Lysobacter enzymogenes
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.1
69000
-
2 * 69000, SDS-PAGE of the heat-denatured enzyme
Lysobacter enzymogenes
3.1.3.1
76000
-
2 * 76000, SDS-PAGE of the enzyme without heat treatment
Lysobacter enzymogenes
3.1.3.1
150000
-
gel filtration
Lysobacter enzymogenes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.1
Lysobacter enzymogenes
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.1
-
Lysobacter enzymogenes
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.3.1
additional information
-
-
Lysobacter enzymogenes
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.1
-22C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several weeks
Lysobacter enzymogenes
3.1.3.1
0-4C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several days
Lysobacter enzymogenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
2',3'-GMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
2',3'-UMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
2'-AMP + H2O
-
94596
Lysobacter enzymogenes
adenosine + phosphate
-
-
-
?
3.1.3.1
2-phosphoglycerate + H2O
-
94596
Lysobacter enzymogenes
glycerol + phosphate
-
-
-
?
3.1.3.1
3'-AMP + H2O
-
94596
Lysobacter enzymogenes
adenosine + phosphate
-
-
-
?
3.1.3.1
3-phosphoglycerate + H2O
-
94596
Lysobacter enzymogenes
glycerol + phosphate
-
-
-
?
3.1.3.1
5'-AMP + H2O
-
94596
Lysobacter enzymogenes
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-CMP + H2O
-
94596
Lysobacter enzymogenes
cytidine + phosphate
-
-
-
?
3.1.3.1
5'-dAMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-dCMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-dGMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-dTMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-GMP + H2O
-
94596
Lysobacter enzymogenes
guanosine + phosphate
-
-
-
?
3.1.3.1
5'-UMP + H2O
-
94596
Lysobacter enzymogenes
uridine + phosphate
-
-
-
?
3.1.3.1
ADP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
ATP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
Glucose 6-phosphate + H2O
-
94596
Lysobacter enzymogenes
Glucose + phosphate
-
-
-
?
3.1.3.1
p-nitrophenyl phosphate + H2O
-
94596
Lysobacter enzymogenes
p-nitrophenol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.1
dimer
2 * 69000, SDS-PAGE of the heat-denatured enzyme; 2 * 76000, SDS-PAGE of the enzyme without heat treatment
Lysobacter enzymogenes
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.3.1
60
-
stable for at least 20 min
Lysobacter enzymogenes
3.1.3.1
70
-
30% loss of activity after 10 min, 98% loss of activity after 20 min
Lysobacter enzymogenes
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.1
8.5
-
-
Lysobacter enzymogenes
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.1
8-hydroxyquinoline
-
Lysobacter enzymogenes
3.1.3.1
EDTA
-
Lysobacter enzymogenes
3.1.3.1
phosphate
insensitive
Lysobacter enzymogenes
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.1
0.034
-
p-nitrophenyl phosphate
and a second Km value of 0.056 mM
Lysobacter enzymogenes
3.1.3.1
0.056
-
p-nitrophenyl phosphate
and a second Km value of 0.034 mM
Lysobacter enzymogenes
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.1
membrane
enzyme is associated with the outer membrane
Lysobacter enzymogenes
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.1
69000
-
2 * 69000, SDS-PAGE of the heat-denatured enzyme
Lysobacter enzymogenes
3.1.3.1
76000
-
2 * 76000, SDS-PAGE of the enzyme without heat treatment
Lysobacter enzymogenes
3.1.3.1
150000
-
gel filtration
Lysobacter enzymogenes
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.1
-
Lysobacter enzymogenes
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.3.1
additional information
-
-
Lysobacter enzymogenes
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.1
-22C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several weeks
Lysobacter enzymogenes
3.1.3.1
0-4C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several days
Lysobacter enzymogenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.1
2',3'-GMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
2',3'-UMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
2'-AMP + H2O
-
94596
Lysobacter enzymogenes
adenosine + phosphate
-
-
-
?
3.1.3.1
2-phosphoglycerate + H2O
-
94596
Lysobacter enzymogenes
glycerol + phosphate
-
-
-
?
3.1.3.1
3'-AMP + H2O
-
94596
Lysobacter enzymogenes
adenosine + phosphate
-
-
-
?
3.1.3.1
3-phosphoglycerate + H2O
-
94596
Lysobacter enzymogenes
glycerol + phosphate
-
-
-
?
3.1.3.1
5'-AMP + H2O
-
94596
Lysobacter enzymogenes
adenosine + phosphate
-
-
-
?
3.1.3.1
5'-CMP + H2O
-
94596
Lysobacter enzymogenes
cytidine + phosphate
-
-
-
?
3.1.3.1
5'-dAMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-dCMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-dGMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-dTMP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
5'-GMP + H2O
-
94596
Lysobacter enzymogenes
guanosine + phosphate
-
-
-
?
3.1.3.1
5'-UMP + H2O
-
94596
Lysobacter enzymogenes
uridine + phosphate
-
-
-
?
3.1.3.1
ADP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
ATP + H2O
-
94596
Lysobacter enzymogenes
?
-
-
-
?
3.1.3.1
Glucose 6-phosphate + H2O
-
94596
Lysobacter enzymogenes
Glucose + phosphate
-
-
-
?
3.1.3.1
p-nitrophenyl phosphate + H2O
-
94596
Lysobacter enzymogenes
p-nitrophenol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.1
dimer
2 * 69000, SDS-PAGE of the heat-denatured enzyme; 2 * 76000, SDS-PAGE of the enzyme without heat treatment
Lysobacter enzymogenes
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.3.1
60
-
stable for at least 20 min
Lysobacter enzymogenes
3.1.3.1
70
-
30% loss of activity after 10 min, 98% loss of activity after 20 min
Lysobacter enzymogenes
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.1
8.5
-
-
Lysobacter enzymogenes