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Literature summary extracted from

  • Chandran Nair, B.; Majeska, R.J.; Rodan, G.A.
    Rat alkaline phosphatase. I. Purification and characterization of the enzyme from osteosarcoma: generation of monoclonal and polyclonal antibodies (1987), Arch. Biochem. Biophys., 254, 18-27.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 L-homoarginine powerful inhibitor of the bone/liver/kidney isoenzyme and for the osteosarcoma cell enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.8
-
p-nitrophenyl phosphate
-
Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 82000
-
x * 82000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 p-nitrophenyl phosphate + H2O
-
Rattus norvegicus p-nitrophenol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 ? x * 82000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 10.5
-
hydrolysis of p-nitrophenyl phosphate Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 9.2 11 pH 9.2: about 40% of maximal activity, pH 11.0: about 60% of maximal activity Rattus norvegicus