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Literature summary extracted from

  • Söling, H.D.; Rescher, C.
    On the regulation of cold-labile cytosolic and of mitochondrial acetyl-CoA hydrolase in rat liver (1985), Eur. J. Biochem., 147, 111-117.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.1 adenosine 5'-[beta,gamma-imido]triphosphate with adoPP[CH2]P a 2.5fold activation of the cold-labile cytosolic enzyme at 2 mM, with adoPP[NH]P a 3.7fold activation respectively Rattus norvegicus
3.1.2.1 ATP 14fold activation of the cold-labile enzyme at 2 mM Rattus norvegicus
3.1.2.1 CTP counteracts the inhibition of the cold-labile cytosolic enzyme by ADP Rattus norvegicus
3.1.2.1 GTP 4.6fold activation of the cold-labile cytosolic enzyme at 2 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.1 ADP 50% inhibition of the cold-labile cytosolic enzyme at 0.0145 mM Rattus norvegicus
3.1.2.1 ATP 0.5 mM nucleoside phosphate inhibits 49% in Trypanosoma brucei brucei 427 Rattus norvegicus
3.1.2.1 CoA 80% inhibition of the cold-labile cytosolic enzyme at 0.04 mM Rattus norvegicus
3.1.2.1 GDP 50% inhibition of the cold-labile cytosolic enzyme at 0.0145 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.2.1 cytosol
-
 Rattus norvegicus 5829
-
3.1.2.1 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.1 68000
-
 SDS-PAGE gel electrophoresis, the cold labile cytosolic acetyl-CoA hydrolase Rattus norvegicus
3.1.2.1 136000
-
 gel filtration, cytosolic native enzyme in the presence of 2 mM ATP, dimeric form Rattus norvegicus
3.1.2.1 157000
-
 gel filtration, mitochondrial enzyme Rattus norvegicus
3.1.2.1 245000
-
 gel filtration, cytosolic native enzyme in the presence of 2 mM ATP, tetrameric form Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.1 acetyl-CoA + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.1 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.1 acetyl-CoA + H2O
-
 Rattus norvegicus CoA + acetate
-
 ?
3.1.2.1 acetyl-CoA + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.2.1 butyryl-CoA + H2O
-
 Rattus norvegicus butanoate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.1 dimer
-
 Rattus norvegicus
3.1.2.1 tetramer
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.1 0.0145
-
 GDP cold-labile cytosolic enzyme Rattus norvegicus