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Literature summary extracted from

  • Nagasawa, T.; Sugisaki, H.; Tani, Y.; Ogata, K.
    Purification and characterization of butyrylcholine-hydrolyzing enzyme from Pseudomonas polycolor (1976), Biochim. Biophys. Acta, 429, 817-827.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 AgNO3
-
 Pseudomonas aeruginosa
3.1.1.8 atropine competitive Pseudomonas aeruginosa
3.1.1.8 CuSO4
-
 Pseudomonas aeruginosa
3.1.1.8 diisopropylphosphofluoridate irreversible inhibition Pseudomonas aeruginosa
3.1.1.8 HgCl2
-
 Pseudomonas aeruginosa
3.1.1.8 homatropine competitive Pseudomonas aeruginosa
3.1.1.8 iodoacetic acid
-
 Pseudomonas aeruginosa
3.1.1.8 additional information enzyme is inhibited by quaternary ammonium salts. The effect is influenced by the size of the quaternary ammonium ion Pseudomonas aeruginosa
3.1.1.8 p-chloromercuribenzoate
-
 Pseudomonas aeruginosa
3.1.1.8 physostigmine i.e. eserine; irreversible inhibition Pseudomonas aeruginosa
3.1.1.8 procaine competitive Pseudomonas aeruginosa
3.1.1.8 quinidine competitive Pseudomonas aeruginosa
3.1.1.8 Scopolamine competitive Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.7
-
 Butyrylcholine pH 7.5, 25°C Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.8 59000
-
 gel filtration Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Pseudomonas aeruginosa
-
 IFO 3918
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.8
-
 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 additional information
-
-
 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.1.1.8 -20°C, 0.1 M potassium phosphate buffer, pH 7.4, 1 month, no loss of activity Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 benzoylcholine + H2O
-
 Pseudomonas aeruginosa benzoate + choline
-
 ?
3.1.1.8 butyrylcholine + H2O best substrate Pseudomonas aeruginosa butanoate + choline
-
 ?
3.1.1.8 butyrylthiocholine + H2O good substrate Pseudomonas aeruginosa butanoate + thiocholine
-
 ?
3.1.1.8 ethyl n-butyrate + H2O poor substrate Pseudomonas aeruginosa ethanol + n-butanoate
-
 ?
3.1.1.8 isoamyl isovalerate + H2O poor substrate Pseudomonas aeruginosa isopentanol + isopentanoate
-
 ?
3.1.1.8 isoamyl n-butyrate + H2O
-
 Pseudomonas aeruginosa isopentanol + n-butanoate
-
 ?
3.1.1.8 isoamyl propionate + H2O
-
 Pseudomonas aeruginosa isopentanol + propanoate
-
 ?
3.1.1.8 isobutyl isovalerate + H2o poor substrate Pseudomonas aeruginosa isobutanol + isopentanoate
-
 ?
3.1.1.8 isobutyl n-butyrate + H2O
-
 Pseudomonas aeruginosa isobutanol + n-butanoate
-
 ?
3.1.1.8 isobutyl propionate + H2O poor substrate Pseudomonas aeruginosa isobutanol + propanoate
-
 ?
3.1.1.8 methyl n-butyrate + H2O poor substrate Pseudomonas aeruginosa methanol + n-butanoate
-
 ?
3.1.1.8 methyl n-valerate + H2O poor substrate Pseudomonas aeruginosa methanol + n-pentanoate
-
 ?
3.1.1.8 additional information butyryl esters are hydrolyzed at a greater rate than propionyl, pentanoyl, valeryl- or acetyl esters Pseudomonas aeruginosa ?
-
 ?
3.1.1.8 n-amyl acetate + H2O poor substrate Pseudomonas aeruginosa n-pentanol + acetate
-
 ?
3.1.1.8 n-amyl n-butyrate + H2O good substrate Pseudomonas aeruginosa n-pentanol + n-butanoate
-
 ?
3.1.1.8 n-butyl acetate + H2O poor substrate Pseudomonas aeruginosa n-butanol + acetate
-
 ?
3.1.1.8 n-butyl n-butyrate + H2O poor substrate Pseudomonas aeruginosa n-butanol + n-butanoate
-
 ?
3.1.1.8 n-hexyl acetate + H2O poor substrate Pseudomonas aeruginosa n-hexanol + acetate
-
 ?
3.1.1.8 propionylcholine + H2O good substrate Pseudomonas aeruginosa propanoate + choline
-
 ?
3.1.1.8 propionylthiocholine + H2O
-
 Pseudomonas aeruginosa propanoate + thiocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 butyrylcholine-hydrolyzing enzyme
-
 Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.8 70
-
 0.1 M phosphate buffer, pH 5.5, 4% loss of activity Pseudomonas aeruginosa
3.1.1.8 80
-
 above 80°C, 10 min, 0.1 M phosphate buffer, pH 5.5, complete inactivation Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7.5 8 optimum reactivity for hydrolysis of butyrylcholine as substrate Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.8 4.5
-
 5 min, 75°C, 90% loss of activity Pseudomonas aeruginosa
3.1.1.8 5.5 7 5 min, 75°C, stable between Pseudomonas aeruginosa
3.1.1.8 8.5
-
 5 min, 75°C, 60% loss of activity Pseudomonas aeruginosa