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Literature summary extracted from

  • Higa, H.H.; Manzi, A.; Diaz, S.; Varki, A.
    Sialate 9-O-acetylesterase from rat liver (1989), Methods Enzymol., 179, 409-415.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.53 activity becomes unstable on excessive dilution Rattus norvegicus
3.1.1.53 lyophilization and reconstitution results in loss of 30% of activity Rattus norvegicus
3.1.1.53 single cycle of freeze-thaw results in little loss of activity, repeated freeze-thaw results in gradual loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.53 9-acetamido-N-acetyl-O-acetylneuraminate little effect at 5 mM Rattus norvegicus
3.1.1.53 bis-p-nitrophenyl phosphate little effect on the activity Rattus norvegicus
3.1.1.53 CHAPS lowers the activity by 40% Rattus norvegicus
3.1.1.53 deoxycholate 75-80% loss of activity at 2% concentration Rattus norvegicus
3.1.1.53 Diethyl-4-nitrophenylphosphate irreversibly inactivated Rattus norvegicus
3.1.1.53 diisopropyl fluorophosphate irreversibly inactivated Rattus norvegicus
3.1.1.53 Hg2+ little effect on the activity Rattus norvegicus
3.1.1.53 additional information not affected by detergents, i.e. Triton X-100, Triton CF-54, saponin Rattus norvegicus
3.1.1.53 p-chloromercuribenzoate little effect on the activity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.53 Golgi vesicle
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.53 30000
-
 1 * 30000 + 1 * 36000 Rattus norvegicus
3.1.1.53 36000
-
 1 * 30000 + 1 * 36000 Rattus norvegicus
3.1.1.53 61000
-
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.53 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.53 liver acetone powder of the glycosylated form of the enzyme Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.53 0.84
-
 4-methylumbelliferyl acetate as substrate Rattus norvegicus
3.1.1.53 11.6
-
 alpha-naphthyl acetate as substrate Rattus norvegicus
3.1.1.53 12.8
-
 4-nitrophenyl acetate as substrate Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.1.1.53 4°C, concentrated purified enzyme, stable for at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.53 4-nitrophenyl acetate + H2O
-
 Rattus norvegicus 4-nitrophenol + acetate
-
 ?
3.1.1.53 alpha-naphthyl acetate + H2O
-
 Rattus norvegicus 1-naphthol + acetate
-
 ?
3.1.1.53 N-acetyl-9-O-acetylneuraminate + H2O
-
 Rattus norvegicus N-acetylneuraminate + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.53 dimer 1 * 30000 + 1 * 36000 Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.53 sialate 9-O-acetylesterase
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.53 37
-
 40% activity is lost in 20 h Rattus norvegicus