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Literature summary extracted from

  • Higa, H.H.; Manzi, A.; Varki, A.
    O-Acetylation and de-O-acetylation of sialic acids. Purification, characterization, and properties of a glycosylated rat liver esterase specific for 9-O-acetylated sialic acids (1989), J. Biol. Chem., 264, 19435-19442.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.53 lyophilization and reconstitution results in loss of 30% of activity Rattus norvegicus
3.1.1.53 single cycle of freeze-thaw results in little loss of activity, repeated freeze-thaw results in gradual loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.53 2,3-Butanedione irreversibly inactivated Rattus norvegicus
3.1.1.53 9-acetamido-N-acetyl-O-acetylneuraminate no effect up to 5 mM Rattus norvegicus
3.1.1.53 bis-p-nitrophenyl phosphate little effect on the activity Rattus norvegicus
3.1.1.53 CHAPS lowers the activity by 40% Rattus norvegicus
3.1.1.53 deoxycholate 75-80% loss of activity at 2% concentration Rattus norvegicus
3.1.1.53 Diethyl-4-nitrophenylphosphate irreversibly inactivated Rattus norvegicus
3.1.1.53 diisopropyl fluorophosphate irreversibly inactivated Rattus norvegicus
3.1.1.53 F- little effect on the activity Rattus norvegicus
3.1.1.53 Hg2+ little effect on the activity Rattus norvegicus
3.1.1.53 additional information not affected by detergents, i.e. Triton X-100, Triton CF-54, saponin; not affected by EDTA Rattus norvegicus
3.1.1.53 p-chloromercuribenzoate little effect on the activity Rattus norvegicus
3.1.1.53 Phenylglyoxal irreversibly inactivated Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.53 8.8
-
N-acetyl-9-O-acetylneuraminic acid
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.53 microsome
-
Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.53 additional information no divalent cation requirement Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.53 36400
-
1 * 36400 + 1* 30300, SDS-PAGE Rattus norvegicus
3.1.1.53 62800
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.53 additional information Rattus norvegicus O-acetyl esters ?
-
?
3.1.1.53 additional information Rattus norvegicus specific for sialic acids and selectively cleaves acetyl groups at the 9-position. it deacetylates di-O-acetyl- and tri-O-acetyl-N-acetylneuraminic acids by first cleaving the O-acetyl ester at the 9-position. The 7- and 8-acetylesters then undergo spontaneous migration to the 9-position, where they can be cleaved ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.53 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.53 side-chain modification contains N-linked oligosaccarides, after treatment with peptide N-glycosidase decrease of 10000 Da in the apparent molecular weight, endoglycosidase H causes a much smaller shift Rattus norvegicus
3.1.1.53 side-chain modification glycoprotein, linked with N-oligosaccharides, LSE, cleaved by endo-beta-N-acetylglycosaminidase H and peptide:N-glycosidase F Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.53 3000fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.53 brain almost exclusively the nonglycosylated form of the enzyme Rattus norvegicus
-
3.1.1.53 colonic mucosa high levels of activity of both forms of the enzyme, nonglycosylated and glycosylated Rattus norvegicus
-
3.1.1.53 heart almost exclusively the nonglycosylated form of the enzyme Rattus norvegicus
-
3.1.1.53 kidney high levels of activity of both forms, nonglycosylated and glycosylated Rattus norvegicus
-
3.1.1.53 liver acetone powder, equal amounts of the glycosylated and nonglycosylated form of the enzyme Rattus norvegicus
-
3.1.1.53 skeletal muscle only the glycosylated form of the enzyme Rattus norvegicus
-
3.1.1.53 testis high levels of activity of both forms, nonglycosylated and glycosylated Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.53 4°C, concentrated purified enzyme, stable for at least 1 month Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.53 3'-O-acetylthymidine + H2O 8% of the activity compared to N-acetyl-9-O-acetylneuraminate Rattus norvegicus thymidine + acetate
-
?
3.1.1.53 4-methylumbelliferyl butyrate + H2O less than 5% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 Rattus norvegicus 4-methylumbelliferone + butyrate
-
?
3.1.1.53 4-nitrophenyl acetate + H2O 72% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 Rattus norvegicus 4-nitrophenol + acetate
-
?
3.1.1.53 alpha-naphthyl acetate + H2O 65% of the activity compared to 4-methylumbelliferyl acetate at pH 5.5 Rattus norvegicus 1-naphthol + acetate
-
?
3.1.1.53 additional information strict substrate specificity for O-acetylated acids and a broad specificity for small synthetic esters, little or no activity against natural acetylated compounds other than sialic acids Rattus norvegicus ?
-
?
3.1.1.53 additional information O-acetyl esters Rattus norvegicus ?
-
?
3.1.1.53 additional information specific for sialic acids and selectively cleaves acetyl groups at the 9-position. it deacetylates di-O-acetyl- and tri-O-acetyl-N-acetylneuraminic acids by first cleaving the O-acetyl ester at the 9-position. The 7- and 8-acetylesters then undergo spontaneous migration to the 9-position, where they can be cleaved Rattus norvegicus ?
-
?
3.1.1.53 N-acetyl-9-O-acetylneuraminate + H2O
-
Rattus norvegicus N-acetylneuraminate + acetate
-
?
3.1.1.53 O-acetylserine + H2O less than 5% of the activity compared to N-acetyl-9-O-acetylneuraminate Rattus norvegicus serine + acetate
-
?
3.1.1.53 triacetin + H2O 26% of the activity compared to N-acetyl-9-O-acetylneuraminate Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.53 dimer 1 * 36400 + 1* 30300, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.53 sialic acid O-acetylesterase
-
Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.53 37
-
40% activity is lost in 20 h Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.53 6 7.5
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.53 5 8 pH 5: 16% of the activity, pH 8: 80% of the activity Rattus norvegicus