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Literature summary extracted from

  • Moreau, R.A.; Huang, A.H.C.
    Enzymes of wax ester catabolism in jojoba (1981), Methods Enzymol., 71, 804-813.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.48 dithiothreitol 5 mM causes 58% increase in activity Simmondsia chinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.20 additional information no inhibitor: cyanide Simmondsia chinensis
1.2.1.48 Cu2+ 1 mM causes 60% inhibition Simmondsia chinensis
1.2.1.48 p-chloromercuribenzoate 0.1 mM causes complete inhibition Simmondsia chinensis
3.1.1.50 PCMB 0.1 mM, 81% inhibition Simmondsia chinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.20 4
-
 Dodecanol
-
 Simmondsia chinensis
1.2.1.48 0.004
-
 decyl aldehyde
-
 Simmondsia chinensis
1.2.1.48 0.25
-
 NAD+
-
 Simmondsia chinensis
3.1.1.50 0.093
-
 N-methylindoxyl myristate
-
 Simmondsia chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.20 membrane
-
 Simmondsia chinensis 16020
-
1.1.3.20 oil body
-
 Simmondsia chinensis
-
-
1.2.1.48 membrane localized primarily in the membrane of wax bodies Simmondsia chinensis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.48 Mn2+ 1 mM causes 24% increase in activity Simmondsia chinensis
1.2.1.48 additional information CaCl2, MgCl2, NaCl and KCl at concentration of 1 mM exert little effect Simmondsia chinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.20 cis-11-eicosenol + O2 Simmondsia chinensis
-
 cis-11-eicosenal + H2O2
-
 ?
1.1.3.20 docosenol + O2 Simmondsia chinensis
-
 docosenal + H2O2
-
 ?
1.1.3.20 long-chain alcohol + O2 Simmondsia chinensis reaction in wax ester catabolism in the storage wax bodies of jojoba plant during germination ?
-
 ?
1.2.1.48 long-chain aldehyde + NAD+ Simmondsia chinensis
-
 ?
-
 ?
3.1.1.50 additional information Simmondsia chinensis the conversion of wax ester to fatty acids in jojoba involves three enzymes: wax ester hydrolase, fatty alcohol oxidase and fatty aldehyde dehydrogenase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.20 Simmondsia chinensis
-
-
-
1.2.1.48 Simmondsia chinensis
-
 jojoba
-
3.1.1.50 Simmondsia chinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.20 partial Simmondsia chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.48 seed activity absent from dry seed Simmondsia chinensis
-
1.2.1.48 seed increases during germination Simmondsia chinensis
-
3.1.1.50 cotyledon
-
 Simmondsia chinensis
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.20 freezing overnight leads to 50% loss of activity Simmondsia chinensis
1.2.1.48 frozen, several months Simmondsia chinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.20 1-tetradecanol + O2 oxidation at 74% the rate of dodecanol oxidation Simmondsia chinensis tetradecanal + H2O2
-
 ?
1.1.3.20 cis-11-eicosenol + O2
-
 Simmondsia chinensis cis-11-eicosenal + H2O2
-
 ?
1.1.3.20 cis-11-eicosenol + O2 oxidation at 19% the rate of dodecanol oxidation Simmondsia chinensis cis-11-eicosenal + H2O2
-
 ?
1.1.3.20 decanol + O2 oxidation at 51% the rate of dodecanol oxidation Simmondsia chinensis decanal + H2O2
-
 ?
1.1.3.20 docosenol + O2
-
 Simmondsia chinensis docosenal + H2O2
-
 ?
1.1.3.20 docosenol + O2 oxidation at 13% the rate of dodecanol oxidation Simmondsia chinensis docosenal + H2O2
-
 ?
1.1.3.20 dodecanol + O2 i.e. lauryl alcohol, preferred substrate Simmondsia chinensis dodecanal + H2O2
-
 ?
1.1.3.20 hexadecanol + O2 i.e. palmityl or cetyl alcohol, oxidation at 26% the rate of dodecanol oxidation Simmondsia chinensis hexadecanal + H2O2
-
 ?
1.1.3.20 long-chain alcohol + O2 reaction in wax ester catabolism in the storage wax bodies of jojoba plant during germination Simmondsia chinensis ?
-
 ?
1.1.3.20 additional information no substrates: ethanol, palmitic acid Simmondsia chinensis ?
-
 ?
1.1.3.20 octadecanol + O2 i.e. stearyl alcohol, oxidation at 16% the rate of dodecanol oxidation Simmondsia chinensis octadecanal + H2O2
-
 ?
1.2.1.48 decanal + NAD+ + H2O
-
 Simmondsia chinensis decanoic acid + NADH
-
 ?
1.2.1.48 dodecanal + NAD+ + H2O
-
 Simmondsia chinensis dodecanoic acid + NADH
-
 ?
1.2.1.48 hexadecanal + NAD+ + H2O
-
 Simmondsia chinensis hexadecanoic acid + NADH
-
 ?
1.2.1.48 long-chain aldehyde + NAD+
-
 Simmondsia chinensis ?
-
 ?
1.2.1.48 long-chain aldehyde + NAD+ + H2O
-
 Simmondsia chinensis long-chain acid anion + NADH
-
 ?
1.2.1.48 additional information not: acetaldehyde Simmondsia chinensis ?
-
 ?
1.2.1.48 tetradecanal + NAD+ + H2O
-
 Simmondsia chinensis tetradecanoic acid + NADH
-
 ?
3.1.1.50 additional information the conversion of wax ester to fatty acids in jojoba involves three enzymes: wax ester hydrolase, fatty alcohol oxidase and fatty aldehyde dehydrogenase Simmondsia chinensis ?
-
 ?
3.1.1.50 N-methylindoxyl myristate + H2O
-
 Simmondsia chinensis ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.48 55
-
 30 min, 80% loss of activity Simmondsia chinensis
3.1.1.50 40
-
-
 Simmondsia chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.20 9
-
-
 Simmondsia chinensis
1.2.1.48 9
-
-
 Simmondsia chinensis
3.1.1.50 9
-
-
 Simmondsia chinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.20 additional information NAD(P)+ Simmondsia chinensis
1.2.1.48 NAD+ NAD+ is a much better electron acceptor than NADP+, FAD or flavin mononucleotide Simmondsia chinensis