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Literature summary extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Characterization of wax-ester hydrolase from roots of white mustard (Sinapis alba L.) seedlings (1985), Acta Biochim. Pol., 32, 263-269.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.50 deoxycholate
-
 Sinapis alba
3.1.1.50 DTT 5 mM, 40% stimulation Sinapis alba
3.1.1.50 Triton X-100 stimulates Sinapis alba

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.50 0.0045
-
 cholesteryl palmitate
-
 Sinapis alba
3.1.1.50 0.028
-
 hexadecanyl palmitate
-
 Sinapis alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.50 membrane
-
 Sinapis alba 16020
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.50 Sinapis alba
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.50
-
 Sinapis alba

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.50 root seedlings Sinapis alba
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.50 0.074
-
-
 Sinapis alba

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.50 butyl palmitate + H2O 76% of the activity with cholesteryl palmitate Sinapis alba butanol + palmitate
-
 ?
3.1.1.50 cholesteryl palmitate + H2O
-
 Sinapis alba cholesterol + palmitate
-
 ?
3.1.1.50 decanyl palmitate + H2O 84% of the activity with cholesteryl palmitate Sinapis alba decanol + palmitate
-
 ?
3.1.1.50 docosanyl palmitate + H2O 69% of the activity with cholesteryl palmitate Sinapis alba docosanol + palmitate
-
 ?
3.1.1.50 dodecanyl palmitate + H2O 85% of the activity with cholesteryl palmitate Sinapis alba dodecanol + palmitate
-
 ?
3.1.1.50 eicosanyl palmitate + H2O 72% of the activity with cholesteryl palmitate Sinapis alba eicosanol + palmitate
-
 ?
3.1.1.50 ethyl palmitate + H2O 69% of the activity with cholesteryl palmitate Sinapis alba ethanol + palmitate
-
 ?
3.1.1.50 hexadecanyl acetate + H2O 3% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + acetate
-
 ?
3.1.1.50 hexadecanyl arachidate + H2O 27% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + arachidate
-
 ?
3.1.1.50 hexadecanyl behenic acid ester + H2O 21% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + behenic acid
-
 ?
3.1.1.50 hexadecanyl butyrate + H2O 10% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + butyrate
-
 ?
3.1.1.50 hexadecanyl caprylate + H2O 25% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + caprylate
-
 ?
3.1.1.50 hexadecanyl laurate + H2O 32% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + laurate
-
 ?
3.1.1.50 hexadecanyl myristate + H2O 36% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + myristate
-
 ?
3.1.1.50 hexadecanyl palmitate + H2O
-
 Sinapis alba hexadecanol + palmitate
-
 ?
3.1.1.50 hexadecanyl palmitate + H2O 77% of the activity with cholesteryl palmitate Sinapis alba hexadecanol + palmitate
-
 ?
3.1.1.50 hexadecanyl stearate + H2O 48% of the activity with hexadecanyl palmitate Sinapis alba hexadecanol + stearate
-
 ?
3.1.1.50 octadecanyl palmitate + H2O 72% of the activity with cholesteryl palmitate Sinapis alba octadecanol + palmitate
-
 ?
3.1.1.50 octanyl palmitate + H2O 92% of the activity with cholesteryl palmitate Sinapis alba octanol + palmitate
-
 ?
3.1.1.50 tetraeicosanyl palmitate + H2O 61% of the activity with cholesteryl palmitate Sinapis alba tetraeicosanol + palmitate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.50 5.8 8.5 broad optimum without definite maximum peak Sinapis alba

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.50 5.5 9 activity sharply decreases below pH 5.5. and above pH 9.0 Sinapis alba