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Literature summary extracted from

  • De Jong, J.G.N.; van den Bosch, H.; Rijken, D.; van Deenen, L.L.M.
    Studies on lysophospholipases. III. The complete purification of two proteins with lysophospholipase activity from beef liver (1974), Biochim. Biophys. Acta, 369, 50-63.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 bis(p-nitrophenyl)phosphate 0.01 mM, 8% inhibition of lysophospholipase I and 61% inhibition of lysophospholipase II Bos taurus
3.1.1.5 deoxycholate
-
 Bos taurus
3.1.1.5 Dithionitrobenzoate
-
 Bos taurus
3.1.1.5 iodoacetamide
-
 Bos taurus
3.1.1.5 NEM
-
 Bos taurus
3.1.1.5 p-hydroxymercuribenzoate
-
 Bos taurus
3.1.1.5 Triton X-100
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 24000
-
 x * 24000, lysophospholipase I, SDS-PAGE Bos taurus
3.1.1.5 26000
-
 lysophospholipase I, SDS-PAGE Bos taurus
3.1.1.5 57000
-
 lysophospholipase II, SDS-PAGE Bos taurus
3.1.1.5 63000
-
 x * 63000, lysophospholipase II, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Bos taurus
-
 lysophospholipase I and lysophospholipase II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.5 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.5 additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 lysophosphatidylcholine + H2O
-
 Bos taurus glycerophosphorylcholine + unesterified fatty acid
-
 ?
3.1.1.5 p-nitrophenyl acetate + H2O
-
 Bos taurus p-nitrophenol + acetate
-
 ?
3.1.1.5 tributyrin + H2O
-
 Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 ? x * 63000, lysophospholipase II, SDS-PAGE Bos taurus
3.1.1.5 ? x * 24000, lysophospholipase I, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 6 8 deacylation of 2-acyllysophosphatidylcholine, lysophospholipase I Bos taurus
3.1.1.5 8.5
-
 deacylation of 2-acyllysophosphatidylcholine, lysophospholipase II Bos taurus