BRENDA - Enzyme Database

Lysophospholipase-transacylase from rat lung

Van den Bosch, H.; Vianen, G.M.; van Heusden, G.P.H.; Methods Enzymol. 71, 513-521 (1981)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.1.5
repeated freezing and thawing causes significant loss of activity
Rattus norvegicus
3.1.1.5
unstable in absence of glycerol and 2-mercaptoethanol
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
diisopropylfluorophosphate
-
Rattus norvegicus
3.1.1.5
iodoacetate
-
Rattus norvegicus
3.1.1.5
NaCN
-
Rattus norvegicus
3.1.1.5
NEM
-
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
58000
-
gel filtration
Rattus norvegicus
3.1.1.5
78000
-
x * 78000, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.5
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.5
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.5
lung
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.5
1.7
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.5
-45°C, less than 10% loss of activity during several months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
-
94378
Rattus norvegicus
palmitic acid + glycero-3-phosphocholine
-
-
-
?
3.1.1.5
lysophosphatidylcholine + H2O
-
94378
Rattus norvegicus
glycerophosphorylcholine + unesterified fatty acid
glycerophosphocholine + fatty acid
94378
Rattus norvegicus
?
3.1.1.5
lysophosphatidylcholine + lysophosphatidylcholine
slight preference for palmitate over stearate and laurate to be incorporated into phosphatidylcholine. No preference for palmitate over myristate
94378
Rattus norvegicus
glycerophosphocholine + phosphatidylcholine
-
94378
Rattus norvegicus
?
3.1.1.5
lysophosphatidylglycerol + H2O
-
94378
Rattus norvegicus
fatty acid + glycerol
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
-
94378
Rattus norvegicus
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylethanolamine + H2O
hydrolysis and acyl transfer
94378
Rattus norvegicus
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.5
?
x * 78000, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
6
7
-
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.5
repeated freezing and thawing causes significant loss of activity
Rattus norvegicus
3.1.1.5
unstable in absence of glycerol and 2-mercaptoethanol
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
diisopropylfluorophosphate
-
Rattus norvegicus
3.1.1.5
iodoacetate
-
Rattus norvegicus
3.1.1.5
NaCN
-
Rattus norvegicus
3.1.1.5
NEM
-
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
58000
-
gel filtration
Rattus norvegicus
3.1.1.5
78000
-
x * 78000, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.5
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.5
lung
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.5
1.7
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.5
-45°C, less than 10% loss of activity during several months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
1-palmitoyl-sn-glycero-3-phosphocholine + H2O
-
94378
Rattus norvegicus
palmitic acid + glycero-3-phosphocholine
-
-
-
?
3.1.1.5
lysophosphatidylcholine + H2O
-
94378
Rattus norvegicus
glycerophosphorylcholine + unesterified fatty acid
glycerophosphocholine + fatty acid
94378
Rattus norvegicus
?
3.1.1.5
lysophosphatidylcholine + lysophosphatidylcholine
slight preference for palmitate over stearate and laurate to be incorporated into phosphatidylcholine. No preference for palmitate over myristate
94378
Rattus norvegicus
glycerophosphocholine + phosphatidylcholine
-
94378
Rattus norvegicus
?
3.1.1.5
lysophosphatidylglycerol + H2O
-
94378
Rattus norvegicus
fatty acid + glycerol
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
-
94378
Rattus norvegicus
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylethanolamine + H2O
hydrolysis and acyl transfer
94378
Rattus norvegicus
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.5
?
x * 78000, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
6
7
-
Rattus norvegicus