BRENDA - Enzyme Database

Purification and some properties of soluble phospholipase B from baker's yeast (Saccharomyces cerevisiae)

Ichimasa, M.; Shiobara, M.; Agric. Biol. Chem. 49, 1083-1089 (1985)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.5
sodium deoxycholate
0.1%, strongly stimulates phospholipase B activity
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
deoxycholate
0.1% sodium deoxycholate, complete inhibition of lysophospholipase activity
Saccharomyces cerevisiae
3.1.1.5
Fe3+
-
Saccharomyces cerevisiae
3.1.1.5
SDS
-
Saccharomyces cerevisiae
3.1.1.5
Triton X-100
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.5
0.05
-
lysophosphatidylcholine
-
Saccharomyces cerevisiae
3.1.1.5
0.63
-
phosphatidylcholine
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.5
membrane
-
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
280000
420000
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.5
Saccharomyces cerevisiae
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
side-chain modification
glycoprotein
Saccharomyces cerevisiae
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.5
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
1-acyl-sn-glycero-3-phosphocholine + H2O
-
94373
Saccharomyces cerevisiae
fatty acid + glycero-3-phosphocholine
-
-
-
?
3.1.1.5
2-acylglycerophosphocholine + H2O
2-acyl-sn-glycero-3-phosphocholine
94373
Saccharomyces cerevisiae
fatty acid + glycerylphosphocholine
-
-
-
?
3.1.1.5
lysophosphatidylcholine + H2O
-
94373
Saccharomyces cerevisiae
glycerophosphorylcholine + unesterified fatty acid
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group
94373
Saccharomyces cerevisiae
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylethanolamine + H2O
-
94373
Saccharomyces cerevisiae
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylinositol + H2O
-
94373
Saccharomyces cerevisiae
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylserine + H2O
-
94373
Saccharomyces cerevisiae
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
3.5
4
-
Saccharomyces cerevisiae
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.5
sodium deoxycholate
0.1%, strongly stimulates phospholipase B activity
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
deoxycholate
0.1% sodium deoxycholate, complete inhibition of lysophospholipase activity
Saccharomyces cerevisiae
3.1.1.5
Fe3+
-
Saccharomyces cerevisiae
3.1.1.5
SDS
-
Saccharomyces cerevisiae
3.1.1.5
Triton X-100
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.5
0.05
-
lysophosphatidylcholine
-
Saccharomyces cerevisiae
3.1.1.5
0.63
-
phosphatidylcholine
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.5
membrane
-
Saccharomyces cerevisiae
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
280000
420000
gel filtration
Saccharomyces cerevisiae
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
side-chain modification
glycoprotein
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.5
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
1-acyl-sn-glycero-3-phosphocholine + H2O
-
94373
Saccharomyces cerevisiae
fatty acid + glycero-3-phosphocholine
-
-
-
?
3.1.1.5
2-acylglycerophosphocholine + H2O
2-acyl-sn-glycero-3-phosphocholine
94373
Saccharomyces cerevisiae
fatty acid + glycerylphosphocholine
-
-
-
?
3.1.1.5
lysophosphatidylcholine + H2O
-
94373
Saccharomyces cerevisiae
glycerophosphorylcholine + unesterified fatty acid
-
-
-
?
3.1.1.5
phosphatidylcholine + H2O
phospholipase B activity, hydrolyzes the acyl ester bonds sequentially, first the 2-acyl group and then the 1-acyl group
94373
Saccharomyces cerevisiae
lysophosphatidylcholine + glycerophosphorylcholine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylethanolamine + H2O
-
94373
Saccharomyces cerevisiae
lysophosphatidylethanolamine + glycerophosphorylethanolamine + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylinositol + H2O
-
94373
Saccharomyces cerevisiae
lysophosphatidylinositol + glycerophosphorylinositol + a carboxylate
-
-
-
?
3.1.1.5
phosphatidylserine + H2O
-
94373
Saccharomyces cerevisiae
lysophosphatidylserine + glycerophosphorylserine + a carboxylate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
3.5
4
-
Saccharomyces cerevisiae