BRENDA - Enzyme Database

A membrane-bound phospholipase A1 purified from Escherichia coli

Scandella, C.J.; Kornberg, A.; Biochemistry 10, 4447-4456 (1971)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.1.32
stable in 3% SDS
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
SDS
Triton enhances the inhibitory effect
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.32
0.0003
-
phosphatidylglycerol
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
plasma membrane
bound to
Escherichia coli
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
Ca2+
required
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
29000
-
x * 29000, SDS-PAGE
Escherichia coli
3.1.1.32
60000
-
sucrose density gradient sedimentation
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.32
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidylcholine + H2O
hydrolysis of L-phosphatidylcholine and not D-phosphatidylcholine
94324
Escherichia coli
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
94324
Escherichia coli
?
-
-
-
?
3.1.1.32
phosphatidylglycerol + H2O
-
94324
Escherichia coli
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 29000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
8.4
-
-
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.32
5.2
8.4
pH 5.2: 10% of maximal activity, pH 6.5: about 50% of maximal activity, pH 8.4: optimum
Escherichia coli
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.1.32
stable in 3% SDS
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
SDS
Triton enhances the inhibitory effect
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.32
0.0003
-
phosphatidylglycerol
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
plasma membrane
bound to
Escherichia coli
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
Ca2+
required
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
29000
-
x * 29000, SDS-PAGE
Escherichia coli
3.1.1.32
60000
-
sucrose density gradient sedimentation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
phosphatidylcholine + H2O
hydrolysis of L-phosphatidylcholine and not D-phosphatidylcholine
94324
Escherichia coli
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
94324
Escherichia coli
?
-
-
-
?
3.1.1.32
phosphatidylglycerol + H2O
-
94324
Escherichia coli
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 29000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
8.4
-
-
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.32
5.2
8.4
pH 5.2: 10% of maximal activity, pH 6.5: about 50% of maximal activity, pH 8.4: optimum
Escherichia coli