BRENDA - Enzyme Database

Purification and properties of a membrane-bound phospholipase A1 from Mycobacterium phlei

Nishijima, M.; Akamatsu, Y.; Nojima, S.; J. Biol. Chem. 249, 5658-5667 (1974)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.32
Triton X-100
0.05% w/v causes maximal enhancement of activity; increases activity
Mycolicibacterium phlei
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
Ca2+
10 mM, 20-60% increase in activity
Mycolicibacterium phlei
3.1.1.32
EDTA
10 mM, 30-40% inhibition
Mycolicibacterium phlei
3.1.1.32
Fe2+
5 mM
Mycolicibacterium phlei
3.1.1.32
Fe3+
1 mM, almost complete inhibition
Mycolicibacterium phlei
3.1.1.32
Hg2+
5 mM
Mycolicibacterium phlei
3.1.1.32
lauryldimethylbenzyl ammonium chloride
-
Mycolicibacterium phlei
3.1.1.32
SDS
-
Mycolicibacterium phlei
3.1.1.32
sodium deoxycholate
-
Mycolicibacterium phlei
3.1.1.32
Triton X-100
at high concentration
Mycolicibacterium phlei
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
plasma membrane
tightly bound
Mycolicibacterium phlei
5886
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Mycolicibacterium phlei
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.32
-
Mycolicibacterium phlei
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
additional information
-
-
Mycolicibacterium phlei
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.32
-20░C, stable for at least 1 year
Mycolicibacterium phlei
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
cardiolipin + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
3.1.1.32
phosphatidylglycerol + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
3.7
-
hydrolysis of cardiolipin
Mycolicibacterium phlei
3.1.1.32
4.5
-
hydrolysis of phosphatidylglycerol
Mycolicibacterium phlei
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.32
Triton X-100
0.05% w/v causes maximal enhancement of activity; increases activity
Mycolicibacterium phlei
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
Ca2+
10 mM, 20-60% increase in activity
Mycolicibacterium phlei
3.1.1.32
EDTA
10 mM, 30-40% inhibition
Mycolicibacterium phlei
3.1.1.32
Fe2+
5 mM
Mycolicibacterium phlei
3.1.1.32
Fe3+
1 mM, almost complete inhibition
Mycolicibacterium phlei
3.1.1.32
Hg2+
5 mM
Mycolicibacterium phlei
3.1.1.32
lauryldimethylbenzyl ammonium chloride
-
Mycolicibacterium phlei
3.1.1.32
SDS
-
Mycolicibacterium phlei
3.1.1.32
sodium deoxycholate
-
Mycolicibacterium phlei
3.1.1.32
Triton X-100
at high concentration
Mycolicibacterium phlei
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
plasma membrane
tightly bound
Mycolicibacterium phlei
5886
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
-
Mycolicibacterium phlei
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
additional information
-
-
Mycolicibacterium phlei
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.32
-20░C, stable for at least 1 year
Mycolicibacterium phlei
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
cardiolipin + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
3.1.1.32
phosphatidylglycerol + H2O
-
94320
Mycolicibacterium phlei
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
3.7
-
hydrolysis of cardiolipin
Mycolicibacterium phlei
3.1.1.32
4.5
-
hydrolysis of phosphatidylglycerol
Mycolicibacterium phlei