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Literature summary extracted from

  • Waite, M.; Rao, R.H.; Griffin, H.; Franson, R.; Miller, C.; Sisson, P.; Frye, J.
    Phospholipases A1 from lysosomes and plasma membranes of rat liver (1981), Methods Enzymol., 71, 674-689.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.32 Ca2+ 10 mM CaCl2, 50-60% inhibition of lysosomal enzyme Rattus norvegicus
3.1.1.32 NaCl 100 mM, 20-30% inhibition of lysosomal enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 lysosome
-
 Rattus norvegicus 5764
-
3.1.1.32 plasmalemma
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.32 Ca2+ slight stimulation of solubilized enzyme from plasmalemma Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 32000
-
 x * 32000, lysosomal enzyme, SDS-PAGE Rattus norvegicus
3.1.1.32 75000
-
 x * 75000, enzyme from plasmalemma, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 6.383
-
 lysosomal enzyme Rattus norvegicus
3.1.1.32 20.6
-
 enzyme from plasmalemma Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1-palmitoyl-2-linoleoyl-sn-3-glycerophosphorylethanolamine + H2O
-
 Rattus norvegicus palmitic acid + 2-linoleoyl-sn-3-glycerophosphorylethanolamine
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.1.32 phosphatidylethanolamine + H2O
-
 Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.32 ? x * 32000, lysosomal enzyme, SDS-PAGE Rattus norvegicus
3.1.1.32 ? x * 75000, enzyme from plasmalemma, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 4
-
 lysosomal enzyme Rattus norvegicus
3.1.1.32 8 9 enzyme from plasmalemma Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.32 3 5 50% of maximal activity at pH 3.0 and at pH 5.0, lysosomal enzyme Rattus norvegicus