BRENDA - Enzyme Database

Phospholipases A1 from lysosomes and plasma membranes of rat liver

Waite, M.; Rao, R.H.; Griffin, H.; Franson, R.; Miller, C.; Sisson, P.; Frye, J.; Methods Enzymol. 71, 674-689 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
Ca2+
10 mM CaCl2, 50-60% inhibition of lysosomal enzyme
Rattus norvegicus
3.1.1.32
NaCl
100 mM, 20-30% inhibition of lysosomal enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
lysosome
-
Rattus norvegicus
5764
-
3.1.1.32
plasmalemma
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
Ca2+
slight stimulation of solubilized enzyme from plasmalemma
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
32000
-
x * 32000, lysosomal enzyme, SDS-PAGE
Rattus norvegicus
3.1.1.32
75000
-
x * 75000, enzyme from plasmalemma, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.32
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.32
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
6.383
-
lysosomal enzyme
Rattus norvegicus
3.1.1.32
20.6
-
enzyme from plasmalemma
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
1-palmitoyl-2-linoleoyl-sn-3-glycerophosphorylethanolamine + H2O
-
94315
Rattus norvegicus
palmitic acid + 2-linoleoyl-sn-3-glycerophosphorylethanolamine
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
94315
Rattus norvegicus
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
94315
Rattus norvegicus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 32000, lysosomal enzyme, SDS-PAGE; x * 75000, enzyme from plasmalemma, SDS-PAGE
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
4
-
lysosomal enzyme
Rattus norvegicus
3.1.1.32
8
9
enzyme from plasmalemma
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.32
3
5
50% of maximal activity at pH 3.0 and at pH 5.0, lysosomal enzyme
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.32
Ca2+
10 mM CaCl2, 50-60% inhibition of lysosomal enzyme
Rattus norvegicus
3.1.1.32
NaCl
100 mM, 20-30% inhibition of lysosomal enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.32
lysosome
-
Rattus norvegicus
5764
-
3.1.1.32
plasmalemma
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.32
Ca2+
slight stimulation of solubilized enzyme from plasmalemma
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.32
32000
-
x * 32000, lysosomal enzyme, SDS-PAGE
Rattus norvegicus
3.1.1.32
75000
-
x * 75000, enzyme from plasmalemma, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.32
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.32
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.1.32
6.383
-
lysosomal enzyme
Rattus norvegicus
3.1.1.32
20.6
-
enzyme from plasmalemma
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.32
1-palmitoyl-2-linoleoyl-sn-3-glycerophosphorylethanolamine + H2O
-
94315
Rattus norvegicus
palmitic acid + 2-linoleoyl-sn-3-glycerophosphorylethanolamine
-
-
-
?
3.1.1.32
phosphatidylcholine + H2O
-
94315
Rattus norvegicus
?
-
-
-
?
3.1.1.32
phosphatidylethanolamine + H2O
-
94315
Rattus norvegicus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.32
?
x * 32000, lysosomal enzyme, SDS-PAGE; x * 75000, enzyme from plasmalemma, SDS-PAGE
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.32
4
-
lysosomal enzyme
Rattus norvegicus
3.1.1.32
8
9
enzyme from plasmalemma
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.32
3
5
50% of maximal activity at pH 3.0 and at pH 5.0, lysosomal enzyme
Rattus norvegicus