BRENDA - Enzyme Database

Purification and properties of a lipid acyl-hydrolase from potato tubers

Hirayama, O.; Matsuda, H.; Takeda, H.; Maenaka, K.; Takatsuka, H.; Biochim. Biophys. Acta 384, 127-137 (1975)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
2,4,6-trifluoro-1,3,5-triazine
16% inhibition at 7 mM, 93% inhibition at 74 mM, 98% inhibition at 741 mM
Solanum tuberosum
3.1.1.26
diisopropylfluorophosphate
complete inhibition at 30 mM and 60 mM
Solanum tuberosum
3.1.1.26
additional information
not affected by dimethyl(2-hydroxy-5-nitrobenzyl)-sulfonium bromide, N-bromosuccinimide, p-chloromercuribenzoate
Solanum tuberosum
3.1.1.26
N-Acetylimidazole
30% inhibition at 30fold mol per mol of enzyme, 48% inhibition at 465fold mol per mol of enzyme, complete inhibition at 180000fold mol per mol of enzyme
Solanum tuberosum
3.1.1.26
N-ethylmaleimide
20% inhibition at 30 mM, 39% inhibition at 60 mM
Solanum tuberosum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.38
-
monogalactosyldiacylglycerol
-
Solanum tuberosum
3.1.1.26
1.7
-
phosphatidylcholine
-
Solanum tuberosum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
70000
-
gel filtration
Solanum tuberosum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.26
Solanum tuberosum
-
L. cv. Benimaru
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
tuber
-
Solanum tuberosum
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
acylsterylglucoside + H2O
500% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
62% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94294
Solanum tuberosum
?
-
-
-
-
3.1.1.26
digalactosylmonoacylglycerol + H2O
26% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
lysophosphatidylcholine + H2O
304% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylethanolamine + H2O
71% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylglycerol + H2O
20% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94294
Solanum tuberosum
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94294
Solanum tuberosum
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
68% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidic acid + H2O
16% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
equal activity compared to monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
33% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylglycerol + H2O
151% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylinositol + H2O
36% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
sulfoquinovosyldiaclglycerol + H2O
52% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.26
5
-
monogalactosyldiacylglycerol as substrate, in citrate buffer
Solanum tuberosum
3.1.1.26
8.5
-
phosphatidylcholine as substrate, in Tris-HCl buffer
Solanum tuberosum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
2,4,6-trifluoro-1,3,5-triazine
16% inhibition at 7 mM, 93% inhibition at 74 mM, 98% inhibition at 741 mM
Solanum tuberosum
3.1.1.26
diisopropylfluorophosphate
complete inhibition at 30 mM and 60 mM
Solanum tuberosum
3.1.1.26
additional information
not affected by dimethyl(2-hydroxy-5-nitrobenzyl)-sulfonium bromide, N-bromosuccinimide, p-chloromercuribenzoate
Solanum tuberosum
3.1.1.26
N-Acetylimidazole
30% inhibition at 30fold mol per mol of enzyme, 48% inhibition at 465fold mol per mol of enzyme, complete inhibition at 180000fold mol per mol of enzyme
Solanum tuberosum
3.1.1.26
N-ethylmaleimide
20% inhibition at 30 mM, 39% inhibition at 60 mM
Solanum tuberosum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.38
-
monogalactosyldiacylglycerol
-
Solanum tuberosum
3.1.1.26
1.7
-
phosphatidylcholine
-
Solanum tuberosum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
70000
-
gel filtration
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Solanum tuberosum
-
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
tuber
-
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
acylsterylglucoside + H2O
500% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
62% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94294
Solanum tuberosum
?
-
-
-
-
3.1.1.26
digalactosylmonoacylglycerol + H2O
26% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
lysophosphatidylcholine + H2O
304% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylethanolamine + H2O
71% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
lysophosphatidylglycerol + H2O
20% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94294
Solanum tuberosum
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94294
Solanum tuberosum
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
68% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidic acid + H2O
16% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
equal activity compared to monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
33% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylglycerol + H2O
151% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
phosphatidylinositol + H2O
36% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
3.1.1.26
sulfoquinovosyldiaclglycerol + H2O
52% of the activity with monogalactosyldiacylglycerol
94294
Solanum tuberosum
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.26
5
-
monogalactosyldiacylglycerol as substrate, in citrate buffer
Solanum tuberosum
3.1.1.26
8.5
-
phosphatidylcholine as substrate, in Tris-HCl buffer
Solanum tuberosum