BRENDA - Enzyme Database

Enzymatic properties of a galactolipase from rice bran

Matsuda, H.; Hirayama, O.; Agric. Biol. Chem. 43, 697-703 (1979)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
2-mercaptoethanol
traces of activity remain with 10 mM at pH 7.5
Oryza sativa
3.1.1.26
CaCl2
79% inhibition at a concentration of 10 mM
Oryza sativa
3.1.1.26
cetyltrimethylammonium chloride
65% inhibition at 1 mM
Oryza sativa
3.1.1.26
cysteine
traces of activity remain with 100 mM at pH 7.5
Oryza sativa
3.1.1.26
diisopropylfluorophosphate
7% remaining activity with 1 mM at pH 7.5
Oryza sativa
3.1.1.26
EDTA
no inhibition at 1 mM, 78% inhibition at 10 mM
Oryza sativa
3.1.1.26
additional information
not affected by 0.3 mM p-chloromercuribenzoate, and 30 mM N-ethylmaleimide
Oryza sativa
3.1.1.26
SDS
0.1% complete inhibition
Oryza sativa
3.1.1.26
Triton X-100
31% inhibition at 0.1%, 83% inhibition at 1%
Oryza sativa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.34
-
monogalactosyldiacylglycerol
-
Oryza sativa
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.26
CaCl2
124% activity compared to assay without additives at 0.1 mM
Oryza sativa
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
40000
-
gel filtration
Oryza sativa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Oryza sativa
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Oryza sativa
-
?
-
-
-
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.1.26
Acetone
complete inhibition at 1%
Oryza sativa
3.1.1.26
Ethanol
complete inhibition at 1%
Oryza sativa
3.1.1.26
ether
complete inhibition at 1%
Oryza sativa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.26
Oryza sativa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
leaf
-
Oryza sativa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
169% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94292
Oryza sativa
?
-
-
-
-
3.1.1.26
lysophosphatidylcholine + H2O
33% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94292
Oryza sativa
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94292
Oryza sativa
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
111% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
27% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
traces of the activity with monogalactosyldiacylglacerol
94292
Oryza sativa
?
-
-
-
?
3.1.1.26
triacylglycerol + H2O
traces of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
?
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.26
35
-
-
Oryza sativa
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.1.26
-
45
0°C: 50% of the maximal activity, 45°C: 40% of the maximal activity
Oryza sativa
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.26
50
-
incubation for 10 min at pH 7.5, 95% loss of activity, retains more thant 60% of its activity at pH 5.5
Oryza sativa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.26
7.5
-
Tris-HCl buffer
Oryza sativa
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.26
4
9
pH 4: 25% of the maximal activity, pH 9: 50% of the maximal activity
Oryza sativa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
2-mercaptoethanol
traces of activity remain with 10 mM at pH 7.5
Oryza sativa
3.1.1.26
CaCl2
79% inhibition at a concentration of 10 mM
Oryza sativa
3.1.1.26
cetyltrimethylammonium chloride
65% inhibition at 1 mM
Oryza sativa
3.1.1.26
cysteine
traces of activity remain with 100 mM at pH 7.5
Oryza sativa
3.1.1.26
diisopropylfluorophosphate
7% remaining activity with 1 mM at pH 7.5
Oryza sativa
3.1.1.26
EDTA
no inhibition at 1 mM, 78% inhibition at 10 mM
Oryza sativa
3.1.1.26
additional information
not affected by 0.3 mM p-chloromercuribenzoate, and 30 mM N-ethylmaleimide
Oryza sativa
3.1.1.26
SDS
0.1% complete inhibition
Oryza sativa
3.1.1.26
Triton X-100
31% inhibition at 0.1%, 83% inhibition at 1%
Oryza sativa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.34
-
monogalactosyldiacylglycerol
-
Oryza sativa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.26
CaCl2
124% activity compared to assay without additives at 0.1 mM
Oryza sativa
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
40000
-
gel filtration
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Oryza sativa
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Oryza sativa
-
?
-
-
-
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.1.26
Acetone
complete inhibition at 1%
Oryza sativa
3.1.1.26
Ethanol
complete inhibition at 1%
Oryza sativa
3.1.1.26
ether
complete inhibition at 1%
Oryza sativa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
leaf
-
Oryza sativa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
169% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94292
Oryza sativa
?
-
-
-
-
3.1.1.26
lysophosphatidylcholine + H2O
33% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94292
Oryza sativa
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94292
Oryza sativa
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
111% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
27% of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
traces of the activity with monogalactosyldiacylglacerol
94292
Oryza sativa
?
-
-
-
?
3.1.1.26
triacylglycerol + H2O
traces of the activity with monogalactosyldiacylglycerol
94292
Oryza sativa
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.26
35
-
-
Oryza sativa
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.1.1.26
-
45
0°C: 50% of the maximal activity, 45°C: 40% of the maximal activity
Oryza sativa
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.1.26
50
-
incubation for 10 min at pH 7.5, 95% loss of activity, retains more thant 60% of its activity at pH 5.5
Oryza sativa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.26
7.5
-
Tris-HCl buffer
Oryza sativa
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.26
4
9
pH 4: 25% of the maximal activity, pH 9: 50% of the maximal activity
Oryza sativa