BRENDA - Enzyme Database

Purification of a lipolytic acyl-hydrolase from Phaseolus vulgaris leaves by affinity chromatography on palmitoylated gauze and its properties.

Matsuda, H.; Tanaka, G.; Morita, K.; Hirayama, O.; Agric. Biol. Chem. 43, 563-570 (1979)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.26
EDTA
212% activity compared to assay without additives at 0.1 mM, 198% at 1 mM, 161% at 10 mM
Phaseolus vulgaris
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
CaCl2
no inhibition at 0.1 and 1.0 mM, 48% inhibition at 10 mM
Phaseolus vulgaris
3.1.1.26
additional information
not inhibited by MgCl2 at 10 mM
Phaseolus vulgaris
3.1.1.26
NaCl
86% inhibition at 50 mM
Phaseolus vulgaris
3.1.1.26
SDS
0.1% complete inhibition
Phaseolus vulgaris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.24
-
monogalactosyldiacylglycerol
-
Phaseolus vulgaris
3.1.1.26
0.53
-
phosphatidylcholine
-
Phaseolus vulgaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
90000
-
SDS-PAGE, gel filtration
Phaseolus vulgaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Phaseolus vulgaris
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Phaseolus vulgaris
-
?
-
-
-
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.1.26
Acetone
88% inhibition at 10%
Phaseolus vulgaris
3.1.1.26
Ethanol
98% inhibition at 10%
Phaseolus vulgaris
3.1.1.26
ether
94% inhibition at 10%
Phaseolus vulgaris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.26
Phaseolus vulgaris
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.26
227fold
Phaseolus vulgaris
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
leaf
-
Phaseolus vulgaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.1.26
4.655
-
-
Phaseolus vulgaris
Storage Stability
EC Number
Storage Stability
Organism
3.1.1.26
-20C, purified enzyme preparation, 3 months stable
Phaseolus vulgaris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
39% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94291
Phaseolus vulgaris
?
-
-
-
-
3.1.1.26
digalactosylmonoacylglycerol + H2O
49% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
lysophosphatidylcholine + H2O
89% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
lysophosphatidylethanolamine + H2O
32% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
lysophosphatidylglycerol + H2O
93% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
best substrate
94291
Phaseolus vulgaris
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94291
Phaseolus vulgaris
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
132% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidic acid + H2O
7% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
31% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
11% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
phosphatidylglycerol + H2O
47% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
sulfoquinovosyldiaclglycerol + H2O
14% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
triacylglycerol + H2O
below 0.1% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.1.26
35
-
monogalactosyldiaylglycerol and phosphatidylcholine as substrates
Phaseolus vulgaris
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.1.26
65
-
inactivated, pH 4 after 20 min, 80% of the activity retained by the same heating at pH 7.5
Phaseolus vulgaris
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.26
EDTA
212% activity compared to assay without additives at 0.1 mM, 198% at 1 mM, 161% at 10 mM
Phaseolus vulgaris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.26
CaCl2
no inhibition at 0.1 and 1.0 mM, 48% inhibition at 10 mM
Phaseolus vulgaris
3.1.1.26
additional information
not inhibited by MgCl2 at 10 mM
Phaseolus vulgaris
3.1.1.26
NaCl
86% inhibition at 50 mM
Phaseolus vulgaris
3.1.1.26
SDS
0.1% complete inhibition
Phaseolus vulgaris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.26
0.24
-
monogalactosyldiacylglycerol
-
Phaseolus vulgaris
3.1.1.26
0.53
-
phosphatidylcholine
-
Phaseolus vulgaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.26
90000
-
SDS-PAGE, gel filtration
Phaseolus vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
Phaseolus vulgaris
-
?
-
-
-
3.1.1.26
monogalactosyldiacylglycerol + H2O
Phaseolus vulgaris
-
?
-
-
-
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.1.26
Acetone
88% inhibition at 10%
Phaseolus vulgaris
3.1.1.26
Ethanol
98% inhibition at 10%
Phaseolus vulgaris
3.1.1.26
ether
94% inhibition at 10%
Phaseolus vulgaris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.26
227fold
Phaseolus vulgaris
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.26
leaf
-
Phaseolus vulgaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.1.26
4.655
-
-
Phaseolus vulgaris
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.1.26
-20C, purified enzyme preparation, 3 months stable
Phaseolus vulgaris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.26
digalactosyldiacylglycerol + H2O
39% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
digalactosyldiacylglycerol + H2O
-
94291
Phaseolus vulgaris
?
-
-
-
-
3.1.1.26
digalactosylmonoacylglycerol + H2O
49% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
digalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
lysophosphatidylcholine + H2O
89% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
lysophosphatidylethanolamine + H2O
32% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
lysophosphatidylglycerol + H2O
93% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
best substrate
94291
Phaseolus vulgaris
galactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
monogalactosyldiacylglycerol + H2O
-
94291
Phaseolus vulgaris
?
-
-
-
-
3.1.1.26
monogalactosylmonoacylglycerol + H2O
132% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
monogalactosylglycerol + fatty acid
-
-
-
?
3.1.1.26
phosphatidic acid + H2O
7% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
phosphatidylcholine + H2O
31% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
phosphatidylethanolamine + H2O
11% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
phosphatidylglycerol + H2O
47% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
sulfoquinovosyldiaclglycerol + H2O
14% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
3.1.1.26
triacylglycerol + H2O
below 0.1% of the activity with monogalactosyldiacylglycerol
94291
Phaseolus vulgaris
?
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.1.26
35
-
monogalactosyldiaylglycerol and phosphatidylcholine as substrates
Phaseolus vulgaris
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.1.1.26
65
-
inactivated, pH 4 after 20 min, 80% of the activity retained by the same heating at pH 7.5
Phaseolus vulgaris