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Literature summary extracted from

  • Fishbein, W.N.; Bessman, S.P.
    Purification and properties of an enzyme in human blood and rat liver microsomes catalyzing the formation and hydrolysis of gamma-lactones. II. Metal ion effects, kinetics, and equilibria (1966), J. Biol. Chem., 241, 4842-4847.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.1.25 Ca2+, 1 mM, stabilizes Homo sapiens
3.1.1.25 Ca2+, 1 mM, stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.25 Mg2+ only inhibition of hydrolytic activity without affecting its lactonizing function Homo sapiens
3.1.1.25 Mg2+ only inhibition of hydrolytic activity without affecting its lactonizing function Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.25 0.9
-
 Gamma-caprolactone
-
 Homo sapiens
3.1.1.25 1.3
-
 Gamma-octalactone
-
 Homo sapiens
3.1.1.25 2.2
-
 Gamma-caprolactone
-
 Rattus norvegicus
3.1.1.25 3.1
-
 Gamma-octalactone
-
 Rattus norvegicus
3.1.1.25 3.3
-
 gamma-valerolactone
-
 Homo sapiens
3.1.1.25 4
-
 gamma-valerolactone
-
 Rattus norvegicus
3.1.1.25 4.5
-
 3-hydroxyoctanoate
-
 Homo sapiens
3.1.1.25 5.7
-
 Gamma-hydroxycaproate
-
 Rattus norvegicus
3.1.1.25 6
-
 gamma-butyrolactone
-
 Rattus norvegicus
3.1.1.25 9
-
 Gamma-hydroxyvalerate
-
 Homo sapiens
3.1.1.25 10.4
-
 Gamma-hydroxyvalerate
-
 Homo sapiens
3.1.1.25 13.3
-
 gamma-butyrolactone
-
 Homo sapiens
3.1.1.25 20
-
 Gamma-hydroxyvalerate
-
 Rattus norvegicus
3.1.1.25 140
-
 gamma-hydroxybutyrate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.25 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.25 Ca2+ activates, hydrolysis is maximal at 0.01 mM , lactonization is maximal with 10 mM Homo sapiens
3.1.1.25 Ca2+ activates, hydrolysis is maximal at 0.01 mM , lactonization is maximal with 10 mM Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.25 Homo sapiens
-
-
-
3.1.1.25 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.25 blood plasma
-
 Homo sapiens
-
3.1.1.25 blood plasma
-
 Rattus norvegicus
-
3.1.1.25 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.25 3-hydroxyoctanoate + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.25 gamma-butyrolactone + H2O
-
 Homo sapiens gamma-hydroxybutyrate
-
 r
3.1.1.25 gamma-butyrolactone + H2O
-
 Rattus norvegicus gamma-hydroxybutyrate
-
 r
3.1.1.25 gamma-caprolactone + H2O
-
 Homo sapiens gamma-hydroxycaproate
-
 ?
3.1.1.25 gamma-caprolactone + H2O
-
 Rattus norvegicus gamma-hydroxycaproate
-
 ?
3.1.1.25 gamma-octalactone + H2O
-
 Homo sapiens 4-hydroxyoctanoate
-
 ?
3.1.1.25 gamma-octalactone + H2O
-
 Rattus norvegicus 4-hydroxyoctanoate
-
 ?
3.1.1.25 gamma-valerolactone + H2O
-
 Homo sapiens gamma-hydroxyvalerate
-
 ?
3.1.1.25 gamma-valerolactone + H2O
-
 Rattus norvegicus gamma-hydroxyvalerate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.25 6
-
-
 Homo sapiens
3.1.1.25 6
-
-
 Rattus norvegicus
3.1.1.25 8.6
-
-
 Homo sapiens
3.1.1.25 8.6
-
-
 Rattus norvegicus