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Literature summary extracted from

  • Yeh, W.K.; Ornston, L.N.
    p-Chloromercuribenzoate specifically modifies thiols associated with the active sites of beta-ketoadipate enol-lactone hydrolase and succinyl CoA: beta-ketoadipate CoA transferase (1984), Arch. Microbiol., 138, 102-105.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.3.6 stable for 1h at 22°C in the presence of 0.00092 mM DTT Acinetobacter calcoaceticus
2.8.3.6 unstable in the absence of sulfhydryl compounds, DTT stabilizes, 80% residual activity after 5h at 0°C in 0.00086 mM DTT Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.6 4-chloromercuribenzoate modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores Acinetobacter calcoaceticus
2.8.3.6 4-chloromercuribenzoate modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores Pseudomonas putida
3.1.1.24 3-oxoadipate 50% inhibition at a concentration of 17 mM; i.e. beta-ketoadipate Acinetobacter calcoaceticus
3.1.1.24 3-oxoadipate 10% inhibition at a concentration of 1 mM; 50% inhibition at a concentration of 10 mM; i.e. beta-ketoadipate Pseudomonas putida
3.1.1.24 5,5'-dithiobis(2-nitrobenzoic acid) completely inactivated, activity can not be recovered by adding dithiothreitol Pseudomonas putida
3.1.1.24 additional information not inhibited by adipate and levulinate at a concentration of 20 mM Acinetobacter calcoaceticus
3.1.1.24 additional information not inhibited by adipate and levulinate at a concentration of 20 mM Pseudomonas putida
3.1.1.24 p-chloromercuribenzoate
-
Acinetobacter calcoaceticus
3.1.1.24 p-chloromercuribenzoate completely inactivated at a concentration of 0.005 mM, 95% of the activity can be recovered by addition of 10 mM dithiothreitol in the reaction mixture; inhibition can not be prevented in the presence of beta-ketoadipate Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O Pseudomonas putida the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase ?
-
?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O Acinetobacter calcoaceticus the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.6 Acinetobacter calcoaceticus
-
mutant strain ADP152, 2 transferases, I: induced by protocatechuate, II: induced by cis,cis-muconate
-
2.8.3.6 Pseudomonas putida
-
-
-
3.1.1.24 Acinetobacter calcoaceticus
-
-
-
3.1.1.24 Pseudomonas putida
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.8.3.6 0°C, in the presence of DTT, 20% loss of activity within 5 h Pseudomonas putida
2.8.3.6 0°C, in the presence of DTT, 20% loss of activity within 5 h Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.6 succinyl-CoA + 3-oxoadipate
-
Pseudomonas putida succinate + 3-oxoadipyl-CoA
-
?
2.8.3.6 succinyl-CoA + 3-oxoadipate
-
Acinetobacter calcoaceticus succinate + 3-oxoadipyl-CoA
-
?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Pseudomonas putida 3-oxoadipate
-
?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Acinetobacter calcoaceticus 3-oxoadipate
-
?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase Pseudomonas putida ?
-
?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase Acinetobacter calcoaceticus ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.3.6 22
-
1 h, stable in the presence of DTT Acinetobacter calcoaceticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.24 10
-
3-oxoadipate
-
Pseudomonas putida
3.1.1.24 17
-
3-oxoadipate
-
Acinetobacter calcoaceticus