EC Number | General Stability | Organism |
---|---|---|
2.8.3.6 | stable for 1h at 22°C in the presence of 0.00092 mM DTT | Acinetobacter calcoaceticus |
2.8.3.6 | unstable in the absence of sulfhydryl compounds, DTT stabilizes, 80% residual activity after 5h at 0°C in 0.00086 mM DTT | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.3.6 | 4-chloromercuribenzoate | modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores | Acinetobacter calcoaceticus | |
2.8.3.6 | 4-chloromercuribenzoate | modifies selectively cysteinyl side chains at or near the active site, one molecule per alphabeta-protomer inactivates, DTT restores | Pseudomonas putida | |
3.1.1.24 | 3-oxoadipate | 50% inhibition at a concentration of 17 mM; i.e. beta-ketoadipate | Acinetobacter calcoaceticus | |
3.1.1.24 | 3-oxoadipate | 10% inhibition at a concentration of 1 mM; 50% inhibition at a concentration of 10 mM; i.e. beta-ketoadipate | Pseudomonas putida | |
3.1.1.24 | 5,5'-dithiobis(2-nitrobenzoic acid) | completely inactivated, activity can not be recovered by adding dithiothreitol | Pseudomonas putida | |
3.1.1.24 | additional information | not inhibited by adipate and levulinate at a concentration of 20 mM | Acinetobacter calcoaceticus | |
3.1.1.24 | additional information | not inhibited by adipate and levulinate at a concentration of 20 mM | Pseudomonas putida | |
3.1.1.24 | p-chloromercuribenzoate | - |
Acinetobacter calcoaceticus | |
3.1.1.24 | p-chloromercuribenzoate | completely inactivated at a concentration of 0.005 mM, 95% of the activity can be recovered by addition of 10 mM dithiothreitol in the reaction mixture; inhibition can not be prevented in the presence of beta-ketoadipate | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | Pseudomonas putida | the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase | ? | - |
? | |
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | Acinetobacter calcoaceticus | the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.6 | Acinetobacter calcoaceticus | - |
mutant strain ADP152, 2 transferases, I: induced by protocatechuate, II: induced by cis,cis-muconate | - |
2.8.3.6 | Pseudomonas putida | - |
- |
- |
3.1.1.24 | Acinetobacter calcoaceticus | - |
- |
- |
3.1.1.24 | Pseudomonas putida | - |
- |
- |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.3.6 | 0°C, in the presence of DTT, 20% loss of activity within 5 h | Pseudomonas putida |
2.8.3.6 | 0°C, in the presence of DTT, 20% loss of activity within 5 h | Acinetobacter calcoaceticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.6 | succinyl-CoA + 3-oxoadipate | - |
Pseudomonas putida | succinate + 3-oxoadipyl-CoA | - |
? | |
2.8.3.6 | succinyl-CoA + 3-oxoadipate | - |
Acinetobacter calcoaceticus | succinate + 3-oxoadipyl-CoA | - |
? | |
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Pseudomonas putida | 3-oxoadipate | - |
? | |
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | 3-oxoadipate enol-lactone | Acinetobacter calcoaceticus | 3-oxoadipate | - |
? | |
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase | Pseudomonas putida | ? | - |
? | |
3.1.1.24 | (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O | the enzyme acts on the product of EC 4.1.1.44 4-carboxymuconolactone decarboxylase | Acinetobacter calcoaceticus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.6 | 22 | - |
1 h, stable in the presence of DTT | Acinetobacter calcoaceticus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.24 | 10 | - |
3-oxoadipate | - |
Pseudomonas putida | |
3.1.1.24 | 17 | - |
3-oxoadipate | - |
Acinetobacter calcoaceticus |