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Literature summary extracted from

  • Somma-Delpero, C.; Valette, A.; Lepetit-Thevenin, J.; Nobili, O.; Boyer, J.; Verine, A.
    Purification and properties of a monoacylglycerol lipase in human erythrocytes (1995), Biochem. J., 312, 519-525.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.23 apolipoprotein A-1
-
 Homo sapiens
3.1.1.23 Diethyl p-nitrophenyl phosphate
-
 Homo sapiens
3.1.1.23 diisopropyl fluorophosphate
-
 Homo sapiens
3.1.1.23 NaCl 1 M, 63% loss of activity Homo sapiens
3.1.1.23 NaF
-
 Homo sapiens
3.1.1.23 PMSF
-
 Homo sapiens
3.1.1.23 SDS
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.23 0.27
-
 1(3)-monooleoylglycerol
-
 Homo sapiens
3.1.1.23 0.47
-
 ethyl oleate
-
 Homo sapiens
3.1.1.23 0.49
-
 sn-2-monooleoylglycerol
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.23 68000
-
 gel filtration Homo sapiens
3.1.1.23 70000
-
 1 * 70000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.23 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.23
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.23 erythrocyte
-
 Homo sapiens
-
3.1.1.23 pancreatic islet
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.1.1.23 4°C, 8 mM CHAPS, 5-7% loss of activity per day Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.23 1-palmitoyl-2-lysophosphatidylcholine + H2O
-
 Homo sapiens palmitic acid + glycerophosphorylcholine
-
 ?
3.1.1.23 2-monoolein + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.23 ethyl oleate + H2O
-
 Homo sapiens oleic acid + ethanol
-
 ?
3.1.1.23 monooleoylglycerol + H2O preferred substrate Homo sapiens oleic acid + glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.23 monomer 1 * 70000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.23 45
-
 15 min, more than 60% loss of activity Homo sapiens
3.1.1.23 55
-
 15 min, complete inactivation Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.23 7.5
-
-
 Homo sapiens