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Literature summary extracted from

  • Nicholson, D.W.; Köhler, H.; Neupert, W.
    Import of cytochrome c into mitochondria. Cytochrome c heme lyase (1987), Eur. J. Biochem., 164, 147-157.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.4.1.17 Cytosolic factor requirement Neurospora crassa
4.4.1.17 glutathione activation Neurospora crassa
4.4.1.17 additional information independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate Neurospora crassa
4.4.1.17 NADH 10fold activation between 1 and 10 mM of NADH Neurospora crassa
4.4.1.17 NADPH about half of the activation observed with NADH Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.17 Deuterohemin r; reversible inhibition Neurospora crassa
4.4.1.17 hemin at concentrations higher than 0.002 mM; at higher concentrations Neurospora crassa
4.4.1.17 N-ethylmaleimide kinetics, in vivo; pretreatment of reticulocyte lysates with N-ethylmaleimide results in nearly complete inactivation Neurospora crassa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.17 mitochondrial inner membrane
-
 Neurospora crassa 5743
-
4.4.1.17 mitochondrion intermembrane space Neurospora crassa 5739
-
4.4.1.17 soluble
-
 Neurospora crassa
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.17 Neurospora crassa
-
 wild-type strain 74A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.17 partial Neurospora crassa
4.4.1.17 partial, subfractionation of mitochondria with digitonin Neurospora crassa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.17 hypha
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.17 additional information
-
 activity is independent of a potential across the inner mitochondrial membrane generated by N,N,N',N'-tetramethyl-p-phenylenediamine/ascorbate, requires an unidentified cytosolic factor Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.17 Apocytochrome c + heme catalyzes covalent attachment of heme group to two cysteine residues in the protein Neurospora crassa Holocytochrome c
-
 ?
4.4.1.17 additional information distinct from apocytochrome c binding protein Neurospora crassa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.17 25
-
-
 Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.17 7
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.17 5.5 8.5 about half-maximal activity at pH 5.5 and 8.5 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.17 NADH activation Neurospora crassa
4.4.1.17 NADPH activation Neurospora crassa