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Literature summary extracted from

  • Walker, E.J.; Treacy, G.B.; Jeffrey, P.D.
    Molecular weights of mitochondrial and cytoplasmic aminoacyl-tRNA synthetases of beef liver and their complexes (1983), Biochemistry, 22, 1934-1941.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 55
-
 2-selenaproline ATP-diphosphate exchange Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.3 cytoplasm
-
 Bos taurus 5737
-
6.1.1.3 mitochondrion
-
 Bos taurus 5739
-
6.1.1.7 cytoplasm
-
 Bos taurus 5737
-
6.1.1.7 mitochondrion
-
 Bos taurus 5739
-
6.1.1.11 cytoplasm
-
 Bos taurus 5737
-
6.1.1.11 mitochondrion
-
 Bos taurus 5739
-
6.1.1.12 cytoplasm
-
 Bos taurus 5737
-
6.1.1.14 cytoplasm
-
 Bos taurus 5737
-
6.1.1.14 mitochondrion
-
 Bos taurus 5739
-
6.1.1.15 cytoplasm
-
 Bos taurus 5737
-
6.1.1.15 mitochondrion
-
 Bos taurus 5739
-
6.1.1.16 cytoplasm
-
 Bos taurus 5737
-
6.1.1.16 mitochondrion
-
 Bos taurus 5739
-
6.1.1.17 cytoplasm
-
 Bos taurus 5737
-
6.1.1.18 mitochondrion
-
 Bos taurus 5739
-
6.1.1.21 cytoplasm
-
 Bos taurus 5737
-
6.1.1.21 mitochondrion
-
 Bos taurus 5739
-
6.1.1.22 cytoplasm
-
 Bos taurus 5737
-
6.1.1.22 mitochondrion
-
 Bos taurus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.3 195000
-
 mitochondrial, gel filtration Bos taurus
6.1.1.3 206000
-
 cytoplasmic, gel filtration Bos taurus
6.1.1.7 141000
-
 gel filtration, cytoplasmic enzyme Bos taurus
6.1.1.7 162000
-
 gel filtration, mitochondrial enzyme Bos taurus
6.1.1.11 additional information
-
-
 Bos taurus
6.1.1.12 117000
-
 gel filtration, also a smaller peak of MW 57000 detected Bos taurus
6.1.1.14 189000
-
 gel filtration, mitochondrial enzyme Bos taurus
6.1.1.14 202000
-
 gel filtration, cytoplasmic enzyme Bos taurus
6.1.1.15 155000
-
 cytoplasmic, gel filtration Bos taurus
6.1.1.15 162000
-
 mitochondrial, gel filtration Bos taurus
6.1.1.16 82000
-
 gel filtration, mitochondrial enzyme Bos taurus
6.1.1.16 262000
-
 gel filtration, cytoplasmic enzyme Bos taurus
6.1.1.17 111000
-
 gel filtration Bos taurus
6.1.1.18 138000
-
 gel filtration Bos taurus
6.1.1.21 153000
-
 gel filtration, cytoplasmic Bos taurus
6.1.1.21 154000
-
 gel filtration, mitochondrial Bos taurus
6.1.1.22 additional information
-
 existence of high molecular weight aminoacyl-tRNA synthetase complexes within the mitochondria and the cytoplasm Bos taurus
6.1.1.22 175000
-
 cytoplasmic enzyme, dissociated activity arises from high molecular weight aminoacyl-tRNA synthetase complex, gel filtration Bos taurus
6.1.1.22 189000
-
 mitochondrial enzyme, dissociated activity arises from high molecular weight aminoacyl-tRNA synthetase complex, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.7 ATP + alanine + tRNAAla Bos taurus key role in protein biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Bos taurus
-
-
-
6.1.1.7 Bos taurus
-
-
-
6.1.1.11 Bos taurus
-
 multienzyme complex containing seryl-tRNA synthetase activity
-
6.1.1.12 Bos taurus
-
-
-
6.1.1.14 Bos taurus
-
-
-
6.1.1.15 Bos taurus
-
-
-
6.1.1.16 Bos taurus
-
-
-
6.1.1.17 Bos taurus
-
-
-
6.1.1.18 Bos taurus
-
-
-
6.1.1.21 Bos taurus
-
-
-
6.1.1.22 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7 partial, from a multienzyme complex containing at least 16 aminoacyl-tRNA synthetase activities Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.3 liver
-
 Bos taurus
-
6.1.1.7 liver
-
 Bos taurus
-
6.1.1.11 liver
-
 Bos taurus
-
6.1.1.12 liver
-
 Bos taurus
-
6.1.1.14 liver
-
 Bos taurus
-
6.1.1.16 liver
-
 Bos taurus
-
6.1.1.17 liver
-
 Bos taurus
-
6.1.1.18 liver
-
 Bos taurus
-
6.1.1.21 liver
-
 Bos taurus
-
6.1.1.22 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Bos taurus AMP + diphosphate + L-threonyl-tRNAThr
-
 ?
6.1.1.7 ATP + alanine + tRNAAla key role in protein biosynthesis Bos taurus ?
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Bos taurus AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.11 ATP + L-serine + tRNASer
-
 Bos taurus AMP + diphosphate + L-seryl-tRNASer
-
 ?
6.1.1.12 ATP + L-aspartate + tRNAAsp
-
 Bos taurus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
6.1.1.14 ATP + glycine + tRNAGly
-
 Bos taurus AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.15 ATP + 2-selenaproline + tRNAPro
-
 Bos taurus ?
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Bos taurus AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Bos taurus AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Bos taurus AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.18 ATP + L-glutamine + tRNAGln
-
 Bos taurus AMP + diphosphate + L-glutaminyl-tRNAGln
-
 ?
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Bos taurus AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Bos taurus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?