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Literature summary extracted from

  • Reitzer, L.J.; Magasanik, B.
    Asparagine synthetases of Klebsiella aerogenes: properties and regulation of synthesis (1982), J. Bacteriol., 151, 1299-1313.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.1 diphosphate
-
 Klebsiella aerogenes
6.3.5.4 5-Chloro-4-oxo-L-norvaline
-
 Klebsiella aerogenes
6.3.5.4 6-diazo-5-oxo-L-norleucine
-
 Klebsiella aerogenes
6.3.5.4 AMP
-
 Klebsiella aerogenes
6.3.5.4 Asn
-
 Klebsiella aerogenes
6.3.5.4 azaserine
-
 Klebsiella aerogenes
6.3.5.4 diphosphate
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.1 0.91
-
 Asp
-
 Klebsiella aerogenes
6.3.5.4 additional information
-
 additional information biphasic kinetic, linear portion near 0.5 Klebsiella aerogenes
6.3.5.4 0.24
-
 ATP ATP Klebsiella aerogenes
6.3.5.4 0.55
-
 Gln
-
 Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.1 80000
-
 gel filtration Klebsiella aerogenes
6.3.5.4 57000
-
 4 * 57000, denaturing PAGE Klebsiella aerogenes
6.3.5.4 230000
-
 gel filtration Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 additional information Klebsiella aerogenes Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.1 Klebsiella aerogenes
-
 the organism contains both enzymes EC 6.3.1.1. and EC 6.3.5.4: The gene asnA codes for ammonia-dependent asparagine synthetases, EC 6.3.1.1, and the gene asnB codes for Gln-dependent asparagine synthetase, EC 6.3.5.4
-
6.3.5.4 Klebsiella aerogenes
-
 the organism contains both enzymes EC 6.3.1.1. and EC 6.3.4.5. The gene asnA codes for NH4+-dependent Asn synthetases, EC 6.3.1.1, and the gene asnB codes for Gln-dependent Asn synthetase, EC 6.3.5.4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.1 additional information
-
-
 Klebsiella aerogenes
6.3.5.4 additional information
-
-
 Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.1 ATP + L-Asp + NH4+
-
 Klebsiella aerogenes AMP + diphosphate + Asn
-
 ?
6.3.5.4 ATP + L-Asp + L-Gln
-
 Klebsiella aerogenes AMP + diphosphate + Asn + Glu
-
 ?
6.3.5.4 ATP + L-Asp + NH3
-
 Klebsiella aerogenes AMP + diphosphate + Asn
-
 ?
6.3.5.4 additional information Gln-dependent enzyme is essential for Asn synthesis when the nitrogen source is growth rate limiting Klebsiella aerogenes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.1 dimer two bands are found in denaturing gel filtration, MW 38000 and MW 42000 Klebsiella aerogenes
6.3.5.4 tetramer 4 * 57000, denaturing PAGE Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 6 8.5 Gln-dependent activity Klebsiella aerogenes