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Literature summary extracted from

  • Kondo, H.; Ohmori, T.; Shibata, H.; Sase, K.; Takahashi, R.; Tokuyama, T.
    Thermostable succinyl-coenzyme A synthetase from Nitrosomonas europaea ATCC 25978: purification and properties (1995), J. Ferment. Bioeng., 79, 499-502.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.5 Cu2+ 0.1 mM Nitrosomonas europaea
6.2.1.5 SDS 1.0 mM Nitrosomonas europaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.5 0.1
-
 CoA
-
 Nitrosomonas europaea
6.2.1.5 0.4
-
 ATP
-
 Nitrosomonas europaea
6.2.1.5 5
-
 succinate succinate Nitrosomonas europaea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.5 Mg2+ 1-100 mM activates Nitrosomonas europaea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.5 29000
-
 2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE Nitrosomonas europaea
6.2.1.5 36000
-
 2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE Nitrosomonas europaea
6.2.1.5 130000
-
 gel filtration Nitrosomonas europaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.5 ADP + phosphate + succinyl-CoA Nitrosomonas europaea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.5 Nitrosomonas europaea
-
 strain ATCC 25978
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.5
-
 Nitrosomonas europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.5 ADP + phosphate + succinyl-CoA
-
 Nitrosomonas europaea ?
-
 ?
6.2.1.5 ATP + succinate + CoA specific for ATP Nitrosomonas europaea ADP + phosphate + succinyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.5 tetramer 2 * 29000 (alpha) + 2 * 36000 (beta), SDS-PAGE Nitrosomonas europaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.5 55
-
-
 Nitrosomonas europaea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.5 50
-
 pH 8.0-10.0, 30 min, stable Nitrosomonas europaea
6.2.1.5 70
-
 pH 7.5, half-life: 60 min Nitrosomonas europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.5 5
-
-
 Nitrosomonas europaea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.2.1.5 8 10 50°C, 30 min stable Nitrosomonas europaea