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Literature summary extracted from

  • Baker, E.N.; Boland, M.J.; Calder, P.C.; Hardman, M.J.
    The specificity of actinidin and its relationship to the structure of the enzyme (1980), Biochim. Biophys. Acta, 616, 30-34.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.14 0.022
-
 benzyloxycarbonyl-Lys p-nitrophenyl ester
-
 Actinidia chinensis
3.4.22.14 0.044
-
 butyloxycarbonyl-Phe-NH-CH2CN
-
 Actinidia chinensis
3.4.22.14 0.12
-
 benzyloxycarbonyl-Gly p-nitrophenyl ester
-
 Actinidia chinensis
3.4.22.14 0.31
-
 benzoyl-Gly p-nitrophenyl ester
-
 Actinidia chinensis
3.4.22.14 1.8
-
 Nalpha-benzoyl-L-Arg p-nitroanilide
-
 Actinidia chinensis
3.4.22.14 21
-
 benzoyl-Lys methyl ester benzoyl-L-Lys methyl ester Actinidia chinensis
3.4.22.14 61
-
 benzoyl-Arg ethyl ester
-
 Actinidia chinensis
3.4.22.14 78
-
 acetyl-Lys methyl ester
-
 Actinidia chinensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.14 Actinidia chinensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.14 acetyl-Gly p-nitrophenyl ester + H2O
-
 Actinidia chinensis acetyl-Gly + 4-nitrophenol
-
 ?
3.4.22.14 acetyl-Gly-Lys methyl ester + H2O
-
 Actinidia chinensis acetyl-Gly-Lys + methanol
-
 ?
3.4.22.14 acetyl-Lys methyl ester + H2O
-
 Actinidia chinensis acetyl-Lys + methanol
-
 ?
3.4.22.14 benzoyl-Arg 4-nitroanilide + H2O
-
 Actinidia chinensis benzoyl-Arg + 4-nitroaniline
-
 ?
3.4.22.14 benzoyl-Arg ethyl ester + H2O
-
 Actinidia chinensis benzoyl-Arg + ethanol
-
 ?
3.4.22.14 benzoyl-Gly p-nitrophenyl ester + H2O
-
 Actinidia chinensis benzoyl-Gly + p-nitrophenol
-
 ?
3.4.22.14 benzoyl-L-Lys methyl ester + H2O
-
 Actinidia chinensis benzoyl-L-Lys + methanol
-
 ?
3.4.22.14 benzyloxycarbonyl 4-nitrophenyl ester + H2O
-
 Actinidia chinensis benzyl formate + 4-nitrophenol
-
 ?
3.4.22.14 benzyloxycarbonyl-Gly p-nitrophenyl ester + H2O
-
 Actinidia chinensis benzyloxycarbonyl-Gly + p-nitrophenol
-
 ?
3.4.22.14 Benzyloxycarbonyl-Lys methyl ester + H2O
-
 Actinidia chinensis Benzyloxycarbonyl-Lys + methanol
-
 ?
3.4.22.14 benzyloxycarbonyl-Lys p-nitrophenyl ester + H2O
-
 Actinidia chinensis benzyloxycarbonyl-Lys + 4-nitrophenol
-
 ?
3.4.22.14 Nalpha-benzoyl-L-Arg p-nitroanilide + H2O
-
 Actinidia chinensis Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
 ?
3.4.22.14 Tosyl-Arg methyl ester + H2O
-
 Actinidia chinensis Tosyl-Arg + methanol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.14 0.029
-
 benzoyl-Arg 4-nitroanilide
-
 Actinidia chinensis
3.4.22.14 0.7
-
 acetyl-Lys methyl ester
-
 Actinidia chinensis
3.4.22.14 3.4
-
 benzyloxycarbonyl 4-nitrophenyl ester
-
 Actinidia chinensis
3.4.22.14 4.5
-
 benzoyl-Arg ethyl ester
-
 Actinidia chinensis
3.4.22.14 5.8
-
 benzoyl-Gly p-nitrophenyl ester
-
 Actinidia chinensis
3.4.22.14 6
-
 Benzyloxycarbonyl-Lys methyl ester
-
 Actinidia chinensis
3.4.22.14 29
-
 benzyloxycarbonyl-Lys p-nitrophenyl ester
-
 Actinidia chinensis