BRENDA - Enzyme Database

Streptococcal proteinase

Liu, T.Y.; Elliott, S.D.; The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ) 3, 609-647 (1971)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.22.10
dithiothreitol
reduction is essential for catalytic activity
Streptococcus sp.
3.4.22.10
thioethanol
reduction is essential for catalytic activity, once the enzyme is activated by the reducing agent, the presence of the activator is no longer necessary for catalytic activity
Streptococcus sp.
3.4.22.10
thioglycolic acid
reduction is essential for catalytic activity
Streptococcus sp.
Crystallization (Commentary)
EC Number
Crystallization
Organism
3.4.22.10
-
Streptococcus sp.
General Stability
EC Number
General Stability
Organism
3.4.22.10
0.1 N HCl or 0.01 N NaOH, room temperature, 2 h, denaturation
Streptococcus sp.
3.4.22.10
0.1 N HCl, 2 h, denaturation of the zymogen
Streptococcus sp.
3.4.22.10
0.5% SDS, denaturation
Streptococcus sp.
3.4.22.10
8 M urea plus reducing agent, 20% of proteolytic activity is provided
Streptococcus sp.
3.4.22.10
EDTA, 0.001 M, stabilizes
Streptococcus sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.22.10
2,4-dinitrofluorobenzene
-
Streptococcus sp.
3.4.22.10
Benzyloxycarbonyl-Gly-Phe
competitive against benzyloxycarbonyl-Phe-Tyr
Streptococcus sp.
3.4.22.10
iodoacetamide
-
Streptococcus sp.
3.4.22.10
iodoacetic acid
-
Streptococcus sp.
3.4.22.10
methyliodine
-
Streptococcus sp.
3.4.22.10
NEM
-
Streptococcus sp.
3.4.22.10
Sodium tetrathionate
-
Streptococcus sp.
3.4.22.10
Tosyl-norleucine-Phe
competitive against benzyloxycarbonyl-norleucine-Phe
Streptococcus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.22.10
additional information
-
additional information
effect of pH-value on the Km-value for benzyloxycarbonyl-Lys-p-nitrophenyl ester
Streptococcus sp.
3.4.22.10
0.22
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.27
Streptococcus sp.
3.4.22.10
0.262
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 6.35
Streptococcus sp.
3.4.22.10
0.494
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.49
Streptococcus sp.
3.4.22.10
2.28
-
benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
2.64
-
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
3.88
-
benzyloxycarbonyl-Glu-Phe
-
Streptococcus sp.
3.4.22.10
5.05
-
Nalpha-benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.22.10
bisulfite
reduction is essential for catalytic activity
Streptococcus sp.
3.4.22.10
cyanide
reduction is essential for catalytic activity
Streptococcus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.22.10
32000
-
high-speed equilibrium ultracentrifugation
Streptococcus sp.
3.4.22.10
33590
-
determination of amino acid composition
Streptococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.22.10
Streptococcus sp.
-
hemolytic
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.4.22.10
side-chain modification
-
Streptococcus sp.
Renatured (Commentary)
EC Number
Commentary
Organism
3.4.22.10
90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold
Streptococcus sp.
Storage Stability
EC Number
Storage Stability
Organism
3.4.22.10
-20°C, stable for at least 2 years
Streptococcus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.10
benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Glu-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
benzyloxycarbonyl-Gly + p-nitrophenol
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Nle-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
N-benzyloxycarbonyl-Gly + 4-nitrophenol
-
-
-
-
3.4.22.10
Nalpha-benzyloxycarbonyl-Lys-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol
-
-
-
?
3.4.22.10
Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.22.10
25
-
0.1 N HCl, 2 h, denaturation of the zymogen
Streptococcus sp.
3.4.22.10
60
-
irreversible inactivation
Streptococcus sp.
3.4.22.10
100
-
pH 6.7, 10 min, denaturation of the zymogen
Streptococcus sp.
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.22.10
additional information
-
additional information
effect of pH-value on the turnover numer for benzyloxycarbonyl-Lys-p-nitrophenyl ester
Streptococcus sp.
3.4.22.10
1.09
-
benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
2.3
-
benzyloxycarbonyl-Glu-Phe
-
Streptococcus sp.
3.4.22.10
5.05
-
Nalpha-benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
8.6
-
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
29.7
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.27
Streptococcus sp.
3.4.22.10
89.8
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.49
Streptococcus sp.
3.4.22.10
142
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 6.35
Streptococcus sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.22.10
4.5
9.5
unstable below pH 4.5 and above pH 9.5
Streptococcus sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.22.10
dithiothreitol
reduction is essential for catalytic activity
Streptococcus sp.
3.4.22.10
thioethanol
reduction is essential for catalytic activity, once the enzyme is activated by the reducing agent, the presence of the activator is no longer necessary for catalytic activity
Streptococcus sp.
3.4.22.10
thioglycolic acid
reduction is essential for catalytic activity
Streptococcus sp.
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.4.22.10
-
Streptococcus sp.
General Stability (protein specific)
EC Number
General Stability
Organism
3.4.22.10
0.1 N HCl or 0.01 N NaOH, room temperature, 2 h, denaturation
Streptococcus sp.
3.4.22.10
0.1 N HCl, 2 h, denaturation of the zymogen
Streptococcus sp.
3.4.22.10
0.5% SDS, denaturation
Streptococcus sp.
3.4.22.10
8 M urea plus reducing agent, 20% of proteolytic activity is provided
Streptococcus sp.
3.4.22.10
EDTA, 0.001 M, stabilizes
Streptococcus sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.22.10
2,4-dinitrofluorobenzene
-
Streptococcus sp.
3.4.22.10
Benzyloxycarbonyl-Gly-Phe
competitive against benzyloxycarbonyl-Phe-Tyr
Streptococcus sp.
3.4.22.10
iodoacetamide
-
Streptococcus sp.
3.4.22.10
iodoacetic acid
-
Streptococcus sp.
3.4.22.10
methyliodine
-
Streptococcus sp.
3.4.22.10
NEM
-
Streptococcus sp.
3.4.22.10
Sodium tetrathionate
-
Streptococcus sp.
3.4.22.10
Tosyl-norleucine-Phe
competitive against benzyloxycarbonyl-norleucine-Phe
Streptococcus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.22.10
additional information
-
additional information
effect of pH-value on the Km-value for benzyloxycarbonyl-Lys-p-nitrophenyl ester
Streptococcus sp.
3.4.22.10
0.22
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.27
Streptococcus sp.
3.4.22.10
0.262
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 6.35
Streptococcus sp.
3.4.22.10
0.494
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.49
Streptococcus sp.
3.4.22.10
2.28
-
benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
2.64
-
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
3.88
-
benzyloxycarbonyl-Glu-Phe
-
Streptococcus sp.
3.4.22.10
5.05
-
Nalpha-benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.22.10
bisulfite
reduction is essential for catalytic activity
Streptococcus sp.
3.4.22.10
cyanide
reduction is essential for catalytic activity
Streptococcus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.22.10
32000
-
high-speed equilibrium ultracentrifugation
Streptococcus sp.
3.4.22.10
33590
-
determination of amino acid composition
Streptococcus sp.
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.4.22.10
side-chain modification
-
Streptococcus sp.
Renatured (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.22.10
90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold
Streptococcus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.4.22.10
-20°C, stable for at least 2 years
Streptococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.10
benzyloxycarbonyl-Glu-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Glu-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
benzyloxycarbonyl-Gly + p-nitrophenol
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Nle-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
benzyloxycarbonyl-Nle-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
N-benzyloxycarbonyl-Gly-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
N-benzyloxycarbonyl-Gly + 4-nitrophenol
-
-
-
-
3.4.22.10
Nalpha-benzyloxycarbonyl-Lys-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
Nalpha-benzyloxycarbonyl-Nepsilon-tosyl-Lys + p-nitrophenol
-
-
-
?
3.4.22.10
Nalpha-benzyloxycarbonyl-Phe-Tyr + H2O
-
81451
Streptococcus sp.
?
-
-
-
?
3.4.22.10
Nepsilon-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
-
81451
Streptococcus sp.
Nepsilon-benzyloxycarbonyl-Lys + 4-nitrophenol
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.22.10
25
-
0.1 N HCl, 2 h, denaturation of the zymogen
Streptococcus sp.
3.4.22.10
60
-
irreversible inactivation
Streptococcus sp.
3.4.22.10
100
-
pH 6.7, 10 min, denaturation of the zymogen
Streptococcus sp.
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.22.10
additional information
-
additional information
effect of pH-value on the turnover numer for benzyloxycarbonyl-Lys-p-nitrophenyl ester
Streptococcus sp.
3.4.22.10
1.09
-
benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
2.3
-
benzyloxycarbonyl-Glu-Phe
-
Streptococcus sp.
3.4.22.10
5.05
-
Nalpha-benzyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
8.6
-
Nalpha-benzyloxycarbonyl-Nepsilon-t-butyloxycarbonyl-Lys-Phe
-
Streptococcus sp.
3.4.22.10
29.7
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.27
Streptococcus sp.
3.4.22.10
89.8
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 4.49
Streptococcus sp.
3.4.22.10
142
-
benzyloxycarbonyl-Lys-p-nitrophenyl ester
pH 6.35
Streptococcus sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.22.10
4.5
9.5
unstable below pH 4.5 and above pH 9.5
Streptococcus sp.