Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miyagawa, E.; Harada, T.; Motoki, Y.
    Purification and properties of N-benzoyl-L-alanine aminohydrolase from Corynebacterium equi (1986), Agric. Biol. Chem., 50, 1527-1531.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.6 1,10-phenanthroline 100% inhibition at 1 mM Rhodococcus equi
3.4.17.6 2-2'-dipyridyl 80% inhibition at 1 mM Rhodococcus equi
3.4.17.6 8-hydroxyquinoline 100% inhibition at 1 mM Rhodococcus equi
3.4.17.6 Ag2+ 100% inhibition at 1 mM Rhodococcus equi
3.4.17.6 Cu2+ 100% inhibition at 1 mM Rhodococcus equi
3.4.17.6 Fe2+ 33% inhibition at 1 mM Rhodococcus equi
3.4.17.6 Fe3+ 47% inhibition at 1 mM Rhodococcus equi
3.4.17.6 Hg2+ 100% inhibition at 1 mM Rhodococcus equi
3.4.17.6 iodoacetic acid 25% inhibition at 1 mM Rhodococcus equi
3.4.17.6 PCMB 100% inhibition at 1 mM Rhodococcus equi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.6 Co2+ 146% activation at 0.5 mM, optimum concentration Rhodococcus equi
3.4.17.6 Mg2+ can partially replace cobalt Rhodococcus equi
3.4.17.6 Ni2+ can partially replace cobalt Rhodococcus equi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.6 40000
-
 6 * 40000, SDS-PAGE Rhodococcus equi
3.4.17.6 230000
-
 gel filtration Rhodococcus equi

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.6 Rhodococcus equi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.6
-
 Rhodococcus equi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.6 0.07
-
-
 Rhodococcus equi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.6 benzoyl-Ala + H2O
-
 Rhodococcus equi benzoate + Ala
-
 ir
3.4.17.6 N-benzoyl-Gly-Ala + H2O
-
 Rhodococcus equi N-benzoyl-Gly + Ala
-
 ir
3.4.17.6 N-benzoyl-L-aminobutyric acid + H2O
-
 Rhodococcus equi benzoate + L-aminobutyric acid
-
 ir
3.4.17.6 p-chlorobenzoyl-Ala + H2O
-
 Rhodococcus equi p-chlorobenzoate + Ala
-
 ir
3.4.17.6 p-chlorobenzoyl-Gly + H2O
-
 Rhodococcus equi p-chlorobenzoate + Gly
-
 ir
3.4.17.6 p-hydroxybenzoyl-Ala + H2O
-
 Rhodococcus equi p-hydroxybenzoate + Ala
-
 ir
3.4.17.6 p-methoxybenzoyl-Gly + H2O
-
 Rhodococcus equi p-methoxybenzoate + Gly
-
 ir
3.4.17.6 p-methoxyenzoyl-Ala + H2O
-
 Rhodococcus equi p-methoxybenzoate + Ala
-
 ir
3.4.17.6 p-nitrobenzoyl-Ala + H2O
-
 Rhodococcus equi p-nitrobenzoate + Ala
-
 ir
3.4.17.6 p-nitrobenzoyl-Gly + H2O
-
 Rhodococcus equi p-nitrobenzoate + Gly
-
 ir

Subunits

EC Number Subunits Comment Organism
3.4.17.6 hexamer 6 * 40000, SDS-PAGE Rhodococcus equi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.6 45
-
-
 Rhodococcus equi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.6 45
-
 below Rhodococcus equi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.6 8
-
-
 Rhodococcus equi