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Literature summary extracted from
- The insulin-secretory-granule carboxypeptidase H. Purification and demonstration of involvement in proinsulin processing (1987), Biochem. J., 245, 575-582.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.10 | 1,10-phenanthroline | - |
Rattus norvegicus |  |
| 3.4.17.10 | CuCl2 | - |
Rattus norvegicus | |
| 3.4.17.10 | EDTA | - |
Rattus norvegicus | |
| 3.4.17.10 | FeSO4 | - |
Rattus norvegicus | |
| 3.4.17.10 | Guanidinoethylmercaptosuccinic acid | - |
Rattus norvegicus | |
| 3.4.17.10 | HgCl2 | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.10 | 0.0175 | - |
dansyl-Phe-Leu-Arg | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.17.10 | secretory granule | - |
Rattus norvegicus | 30141 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.10 | Co2+ | activates | Rattus norvegicus | |
| 3.4.17.10 | NiCl2 | stimulates | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.10 | 55000 | - |
non-denaturing PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.10 | additional information | Rattus norvegicus | biosynthesis of insulin in pancreatic beta-cells | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.10 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.17.10 | side-chain modification | glycoprotein | Rattus norvegicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.10 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.10 | insulinoma cell | - |
Rattus norvegicus | - |
| 3.4.17.10 | pancreas | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.10 | 15.5 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.10 | Dansyl-Phe-Ala-Arg + H2O | - |
Rattus norvegicus | Dansyl-Phe-Ala + Arg | - |
? | |
| 3.4.17.10 | Dansyl-Phe-Leu-Arg + H2O | - |
Rattus norvegicus | Dansyl-Phe-Leu + Arg | - |
? | |
| 3.4.17.10 | additional information | biosynthesis of insulin in pancreatic beta-cells | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.10 | monomer | 1 * 55000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.10 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.10 | 5 | 6 | - |
Rattus norvegicus |
| 3.4.17.10 | 5.5 | - |
- |
Rattus norvegicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.10 | 4 | 6.5 | pH 4: about 20% of activity maximum, pH 6.5: about 15% of activity maximum | Rattus norvegicus |
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Release 2023.1 (January 2023)
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