Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • D'Aniello, A.; Strazullo, L.
    Peptidyl-D-amino acid hydrolase from Loligo vulgaris Lam. Purification and characterization (1984), J. Biol. Chem., 259, 4237-4243.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.17 CaCl2 50 mM, 5-10% inhibition, no inhibition up to 1 mM Loligo vulgaris
3.4.13.17 CdCl2 50 mM, 5-10% inhibition, no inhibition up to 1 mM Loligo vulgaris
3.4.13.17 additional information not inhibitory are 1 mM of MnCl2, MgCl2, ZnCl2, CuCl2 or 100 mM of NaCl, KCl, Na2SO4 or 50 mM of EDTA, guanidine chloride or 3 M of urea Loligo vulgaris
3.4.13.17 Pb(CH3COO)2 50 mM, 5-10% inhibition, no inhibition up to 1 mM Loligo vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.17 2.5
-
 L-Ala-D-Ala
-
 Loligo vulgaris
3.4.13.17 2.8
-
 L-Ala-L-Ala
-
 Loligo vulgaris
3.4.13.17 5.2
-
 Gly-D-Ala
-
 Loligo vulgaris
3.4.13.17 5.4
-
 D-Leu-D-Leu
-
 Loligo vulgaris
3.4.13.17 7.7
-
 Gly-L-Ala
-
 Loligo vulgaris
3.4.13.17 8.6
-
 L-Leu-L-Leu
-
 Loligo vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.17 34000
-
 1 * 106000 + 1 * 34000, SDS-PAGE Loligo vulgaris
3.4.13.17 106000
-
 1 * 106000 + 1 * 34000, SDS-PAGE Loligo vulgaris
3.4.13.17 140000
-
 gel filtration Loligo vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.17 D-amino acid peptide + H2O Loligo vulgaris involved in breakdown of D-amino acid peptides which are present in nervous system of cephalopods ?
-
 ?
3.4.13.17 D-amino acid peptide + H2O Octopus vulgaris involved in breakdown of D-amino acid peptides which are present in nervous system of cephalopods ?
-
 ?
3.4.13.17 D-amino acid peptide + H2O Sepia officinalis involved in breakdown of D-amino acid peptides which are present in nervous system of cephalopods ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.17 Loligo vulgaris
-
-
-
3.4.13.17 Octopus vulgaris
-
-
-
3.4.13.17 Sepia officinalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.17
-
 Loligo vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.17 cecum highest activity in digestive juice Loligo vulgaris
-
3.4.13.17 cecum cecum Loligo vulgaris
-
3.4.13.17 cecum cecum Octopus vulgaris
-
3.4.13.17 cecum cecum Sepia officinalis
-
3.4.13.17 hepatopancreas
-
 Loligo vulgaris
-
3.4.13.17 hepatopancreas
-
 Octopus vulgaris
-
3.4.13.17 hepatopancreas
-
 Sepia officinalis
-
3.4.13.17 kidney
-
 Loligo vulgaris
-
3.4.13.17 kidney
-
 Octopus vulgaris
-
3.4.13.17 kidney
-
 Sepia officinalis
-
3.4.13.17 nervous system
-
 Loligo vulgaris
-
3.4.13.17 nervous system
-
 Octopus vulgaris
-
3.4.13.17 nervous system
-
 Sepia officinalis
-
3.4.13.17 salivary gland posterior Loligo vulgaris
-
3.4.13.17 salivary gland posterior Octopus vulgaris
-
3.4.13.17 salivary gland posterior Sepia officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.17 23.3
-
 substrate Gly-D-Ala Loligo vulgaris

Storage Stability

EC Number Storage Stability Organism
3.4.13.17 -20°C, 0.05 M phosphate buffer, pH 7.0, 50% glycerol, 6 months, little loss of activity Loligo vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.17 D-Ala-D-Ala + H2O 3-20% hydrolysis, depending on reaction time Loligo vulgaris D-Ala + D-Ala
-
 ?
3.4.13.17 D-Ala-Gly + H2O 15-100% hydrolysis, depending on reaction time Loligo vulgaris D-Ala + Gly
-
 ?
3.4.13.17 D-Ala-L-Ala + H2O 1-10% hydrolysis, depending on reaction time Loligo vulgaris D-Ala + L-Ala
-
 ?
3.4.13.17 D-amino acid peptide + H2O involved in breakdown of D-amino acid peptides which are present in nervous system of cephalopods Loligo vulgaris ?
-
 ?
3.4.13.17 D-amino acid peptide + H2O involved in breakdown of D-amino acid peptides which are present in nervous system of cephalopods Octopus vulgaris ?
-
 ?
3.4.13.17 D-amino acid peptide + H2O involved in breakdown of D-amino acid peptides which are present in nervous system of cephalopods Sepia officinalis ?
-
 ?
3.4.13.17 D-Leu-D-Leu + H2O 75-100% hydrolysis, depending on reaction time Loligo vulgaris D-Leu + D-Leu
-
 ?
3.4.13.17 D-Leu-Gly-Gly + H2O 30-100% hydrolysis, depending on reaction time Loligo vulgaris D-Leu + Gly-Gly
-
 ?
3.4.13.17 D-Leu-Leu + H2O 70-100% hydrolysis, depending on reaction time Loligo vulgaris D-Leu + Leu
-
 ?
3.4.13.17 Gly-D-Ala + H2O 80-100% hydrolysis, depending on reaction time Loligo vulgaris Gly + D-Ala
-
 ?
3.4.13.17 Gly-D-Asp + H2O 30-100% hydrolysis, depending on reaction time Loligo vulgaris Gly + D-Asp
-
 ?
3.4.13.17 Gly-D-Leu + H2O 90-100% hydrolysis, depending on reaction time Loligo vulgaris Gly + D-Leu
-
 ?
3.4.13.17 Gly-D-Phe + H2O 10-50% hydrolysis, depending on reaction time Loligo vulgaris Gly + D-Phe
-
 ?
3.4.13.17 Gly-D-Ser + H2O 30-100% hydrolysis, depending on reaction time Loligo vulgaris Gly + D-Ser
-
 ?
3.4.13.17 Gly-Gly-D-Leu + H2O 2-20% hydrolysis, depending on reaction time Loligo vulgaris Gly-Gly + D-Leu
-
 ?
3.4.13.17 Gly-L-Ala + H2O
-
 Loligo vulgaris Gly + L-Ala
-
 ?
3.4.13.17 L-Ala-D-Ala + H2O 100% hydrolysis Loligo vulgaris L-Ala + Ala
-
 ?
3.4.13.17 L-Ala-L-Ala + H2O
-
 Loligo vulgaris L-Ala + L-Ala
-
 ?
3.4.13.17 L-Leu-D-Leu + H2O 2-15% hydrolysis, depending on reaction time Loligo vulgaris Leu + D-Leu
-
 ?
3.4.13.17 L-Leu-L-Leu + H2O
-
 Loligo vulgaris L-Leu + L-Leu
-
 ?
3.4.13.17 L-Tyr-D-Ala-Gly-L-Phe-L-Met-NH2 + H2O 4-40% hydrolysis, depending on reaction time Loligo vulgaris L-Tyr + D-Ala-Gly-L-Phe-L-Met-NH2
-
 ?
3.4.13.17 L-Tyr-D-Arg + H2O 2-20% hydrolysis, depending on reaction time Loligo vulgaris L-Tyr + D-Arg
-
 ?
3.4.13.17 additional information also hydrolysis of L-peptides and enkephalins, no hydrolysis of peptides with substituted amino-terminus Loligo vulgaris ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.17 dimer 1 * 106000 + 1 * 34000, SDS-PAGE Loligo vulgaris
3.4.13.17 More amino acid analysis: high contributions of Asp, Glu, Gly, Val Loligo vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.17 37
-
 assay at Loligo vulgaris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.13.17 20
-
 pH 8.0, stable for 5 days Loligo vulgaris
3.4.13.17 50
-
 pH 8.0, 5 min, 50% loss of activity Loligo vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.17 8
-
-
 Loligo vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.13.17 7.2 8.8
-
 Loligo vulgaris