EC Number | Application | Comment | Organism |
---|---|---|---|
3.4.11.17 | synthesis | part of method for Trp-production | Cutaneotrichosporon cutaneum |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.11.17 | - |
Cutaneotrichosporon cutaneum |
3.5.1.57 | - |
Cutaneotrichosporon cutaneum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.17 | 1,10-phenanthroline | 27% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
3.4.11.17 | alpha,alpha'-dipyridyl | 53% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
3.4.11.17 | monoiodoacetate | 25% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
3.4.11.17 | N-ethylmaleimide | 50% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
3.5.1.57 | 1,10-phenanthroline | weak | Cutaneotrichosporon cutaneum | |
3.5.1.57 | 2,2'-dipyridyl | - |
Cutaneotrichosporon cutaneum | |
3.5.1.57 | monoiodoacetate | weak | Cutaneotrichosporon cutaneum | |
3.5.1.57 | NEM | - |
Cutaneotrichosporon cutaneum | |
3.5.1.57 | PCMB | weak | Cutaneotrichosporon cutaneum | |
3.5.1.57 | PMSF | weak | Cutaneotrichosporon cutaneum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.17 | 5.6 | - |
Trp-amide | - |
Cutaneotrichosporon cutaneum | |
3.5.1.57 | 5.6 | - |
L-Tryptophanamide | - |
Cutaneotrichosporon cutaneum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.17 | cytosol | - |
Cutaneotrichosporon cutaneum | 5829 | - |
3.5.1.57 | cytosol | - |
Cutaneotrichosporon cutaneum | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.17 | Co2+ | can partially replace manganese | Cutaneotrichosporon cutaneum | |
3.4.11.17 | Mg2+ | can partially replace manganese, after long pre-incubation | Cutaneotrichosporon cutaneum | |
3.4.11.17 | Mn2+ | required | Cutaneotrichosporon cutaneum | |
3.5.1.57 | Co2+ | some activation | Cutaneotrichosporon cutaneum | |
3.5.1.57 | Mn2+ | required for activity | Cutaneotrichosporon cutaneum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.17 | 68000 | - |
4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
3.4.11.17 | 270000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
3.5.1.57 | 68000 | - |
4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
3.5.1.57 | 270000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.57 | L-tryptophanamide + H2O | Cutaneotrichosporon cutaneum | - |
L-tryptophan + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.17 | Cutaneotrichosporon cutaneum | - |
- |
- |
3.5.1.57 | Cutaneotrichosporon cutaneum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.17 | - |
Cutaneotrichosporon cutaneum |
3.5.1.57 | - |
Cutaneotrichosporon cutaneum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.17 | 1110 | - |
- |
Cutaneotrichosporon cutaneum |
3.5.1.57 | 1110 | - |
- |
Cutaneotrichosporon cutaneum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.17 | Ala-amide + H2O | - |
Cutaneotrichosporon cutaneum | Ala + NH3 | - |
ir | |
3.4.11.17 | Asn + H2O | - |
Cutaneotrichosporon cutaneum | Asp + NH3 | - |
ir | |
3.4.11.17 | citrulline + H2O | - |
Cutaneotrichosporon cutaneum | ? + ? | - |
ir | |
3.4.11.17 | Gln + H2O | - |
Cutaneotrichosporon cutaneum | Glu + NH3 | - |
ir | |
3.4.11.17 | His-Ala + H2O | - |
Cutaneotrichosporon cutaneum | His + Ala | - |
ir | |
3.4.11.17 | His-Gly + H2O | - |
Cutaneotrichosporon cutaneum | His + Gly | - |
ir | |
3.4.11.17 | His-Leu + H2O | - |
Cutaneotrichosporon cutaneum | His + Leu | - |
ir | |
3.4.11.17 | His-Phe + H2O | - |
Cutaneotrichosporon cutaneum | His + Phe | - |
ir | |
3.4.11.17 | Leu-Ala + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Ala | - |
ir | |
3.4.11.17 | Leu-amide + H2O | - |
Cutaneotrichosporon cutaneum | Leu + NH3 | - |
ir | |
3.4.11.17 | Leu-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Gly | - |
ir | |
3.4.11.17 | Leu-Gly-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Gly-Gly | - |
ir | |
3.4.11.17 | Leu-Phe + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Phe | - |
ir | |
3.4.11.17 | Leu-Trp + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Trp | - |
ir | |
3.4.11.17 | Met-amide + H2O | - |
Cutaneotrichosporon cutaneum | Met + NH3 | - |
ir | |
3.4.11.17 | additional information | generally relatively high affinity towards peptides with N-terminal Trp | Cutaneotrichosporon cutaneum | ? | - |
? | |
3.4.11.17 | Phe-amide + H2O | - |
Cutaneotrichosporon cutaneum | Phe + NH3 | - |
ir | |
3.4.11.17 | Phe-Leu + H2O | - |
Cutaneotrichosporon cutaneum | Phe + Leu | - |
ir | |
3.4.11.17 | Trp-Ala + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Ala | - |
ir | |
3.4.11.17 | Trp-amide + H2O | - |
Cutaneotrichosporon cutaneum | Trp + NH3 | - |
ir | |
3.4.11.17 | Trp-Glu + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Glu | - |
ir | |
3.4.11.17 | Trp-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Gly | - |
ir | |
3.4.11.17 | Trp-Leu + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Leu | - |
ir | |
3.4.11.17 | Trp-Phe + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Phe | - |
ir | |
3.4.11.17 | Trp-Trp + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Trp | - |
ir | |
3.4.11.17 | Trp-Tyr + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Tyr | - |
ir | |
3.4.11.17 | Tyr-amide + H2O | - |
Cutaneotrichosporon cutaneum | Tyr + NH3 | - |
ir | |
3.4.11.17 | Val-Ala + H2O | - |
Cutaneotrichosporon cutaneum | Val + Ala | - |
ir | |
3.4.11.17 | Val-amide + H2O | - |
Cutaneotrichosporon cutaneum | Val + NH3 | - |
ir | |
3.4.11.17 | Val-Gly-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Val + Gly-Gly | - |
ir | |
3.5.1.57 | L-alaninamide + H2O | 14% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-alanine + NH3 | - |
? | |
3.5.1.57 | L-asparagine + H2O | 22% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-aspartate + NH3 | - |
? | |
3.5.1.57 | L-citrullin + H2O | 22% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | ? | - |
? | |
3.5.1.57 | L-glutamine + H2O | 18% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-glutamate + NH3 | - |
? | |
3.5.1.57 | L-leucinamide + H2O | 26% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-leucine + NH3 | - |
? | |
3.5.1.57 | L-methioninamide + H2O | 14% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-methionine + NH3 | - |
? | |
3.5.1.57 | L-phenylalaninamide + H2O | 47% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-phenylalanine + NH3 | - |
? | |
3.5.1.57 | L-serinamide + H2O | 7% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-serine + NH3 | - |
? | |
3.5.1.57 | L-tryptophanamide + H2O | - |
Cutaneotrichosporon cutaneum | L-tryptophan + NH3 | - |
? | |
3.5.1.57 | L-tyrosinamide + H2O | 22% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-tyrosine + NH3 | - |
? | |
3.5.1.57 | L-valinamide + H2O | 15% of the activity with L-tryptophanamide | Cutaneotrichosporon cutaneum | L-valine + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.17 | tetramer | 4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
3.5.1.57 | tetramer | 4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.17 | 40 | 45 | - |
Cutaneotrichosporon cutaneum |
3.5.1.57 | 40 | 45 | crystalline enzyme | Cutaneotrichosporon cutaneum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 35 | 60 | crystalline enzyme, 35°C: about 75% of activity maximum, 60°C: about 35% of activity maximum | Cutaneotrichosporon cutaneum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.17 | 55 | - |
below | Cutaneotrichosporon cutaneum |
3.5.1.57 | 55 | - |
crystalline enzyme, 10 min, stable | Cutaneotrichosporon cutaneum |
3.5.1.57 | 60 | - |
crystalline enzyme, rapid inactivation | Cutaneotrichosporon cutaneum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.17 | 9 | 9.5 | - |
Cutaneotrichosporon cutaneum |
3.5.1.57 | 9 | 9.5 | crystalline enzyme | Cutaneotrichosporon cutaneum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.57 | 7.5 | 8.5 | crystalline enzyme, pH 7.5, 20 h, room temperature | Cutaneotrichosporon cutaneum |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.17 | 1 | - |
N-ethylmaleimide | - |
Cutaneotrichosporon cutaneum |