EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.13 | Co2+ | above 3-7 mM | Hathewaya histolytica | |
3.4.11.13 | CoSO4 | at high concentration, Co-citrate protects | Hathewaya histolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.13 | 0.5 | 3 | L-leucine 4-nitroanilide | - |
Hathewaya histolytica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.13 | Co2+ | required, 0.93-1.25 mol cobalt per mol enzyme, optimal concentration 0.003-0.018 mM, involved in enzyme-substrate binding, inhibitory above 3-7 mM, time-course of activation | Hathewaya histolytica | |
3.4.11.13 | Mn2+ | required | Hathewaya histolytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.13 | Peptides + H2O | Hathewaya histolytica | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.13 | Hathewaya histolytica | - |
soluble and glass- or cellulose-immobilized enzyme | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.11.13 | Release of any N-terminal amino acid, including proline and hydroxyproline, but no cleavage of Xaa-Pro- | release of any N-terminal amino acid, including Pro and hydroxyproline, but no cleavage of XaaPro, mechanism involves Co2+ | Hathewaya histolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.13 | Leu-4-nitroanilide + H2O | - |
Hathewaya histolytica | Leu + 4-nitroaniline | - |
ir | |
3.4.11.13 | Peptides + H2O | - |
Hathewaya histolytica | ? | - |
? | |
3.4.11.13 | Pro-Gly-Pro + H2O | - |
Hathewaya histolytica | Pro + Gly-Pro | - |
ir |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.13 | 4.2 | - |
L-leucine 4-nitroanilide | - |
Hathewaya histolytica |