BRENDA - Enzyme Database

Partial purification and properties of an acid nucleotidase from the postmicrosomal supernatant of rat spleen

Tjernshaugen, H.; Biochem. J. 169, 597-605 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.31
KF
cytosolic enzyme
Rattus norvegicus
3.1.3.31
Tartrate
cytosolic enzyme
Rattus norvegicus
3.1.3.31
Zn2+
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.31
0.7
-
3'-AMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
0.9
-
5'-AMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
1.7
-
3'-dAMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
1.7
-
3'-dGMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
1.7
-
3'-GMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
2.6
-
3'-dTMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
2.6
-
5'-dAMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
3.2
-
5'-dTMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
3.7
-
5'-CMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
4
-
p-nitrophenyl phosphate
cytosolic enzyme
Rattus norvegicus
3.1.3.31
4.7
-
5'-GMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
6.2
-
5'-dCMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
8
-
3'-dUMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
12
-
5'-dGMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
25
-
Glycerol 2-phosphate
cytosolic enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.31
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.31
125000
-
cytosolic enzyme, gel filtration
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.31
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.31
spleen cytosol
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.31
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.31
0.256
-
cytosolic enzyme
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
3.1.3.31
-20°C, 50 mM Tris-HCl buffer, pH 7.5, 1 mM dithiothreitol, 2 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.31
2',3'-cyclic-AMP + H2O
5% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
2'-AMP + H2O
68% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
2-glycerophosphate + H2O
cytosolic enzyme
81066
Rattus norvegicus
glycerol + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-AMP + H2O
-
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-CMP + H2O
-
81066
Rattus norvegicus
cytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
131% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
i.e. 2'-deoxyadenosine 3'-phosphate
81066
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
101% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxycytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
i.e. 2'-deoxycytidine 3'-phosphate
81066
Rattus norvegicus
deoxycytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
77% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
i.e. 2'-deoxythymidine 3'-phosphate
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dUMP + H2O
-
81066
Rattus norvegicus
deoxyuridine + phosphate
-
-
-
-
3.1.3.31
3'-GMP + H2O
49% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
guanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-UMP + H2O
14% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
uridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-AMP + H2O
49% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-CMP + H2O
97% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
cytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dAMP + H2O
128% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
81066
Rattus norvegicus
deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dCMP + H2O
i.e. 2'-deoxycytidine 5'-phosphate
81066
Rattus norvegicus
deoxycytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dGMP + H2O
142% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyguanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
81066
Rattus norvegicus
deoxyguanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dIMP + H2O
46% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyinosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dIMP + H2O
i.e. 2'-deoxyinosine 5'-phosphate
81066
Rattus norvegicus
deoxyinosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
60% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dUMP + H2O
28% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyuridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
81066
Rattus norvegicus
deoxyuridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-GMP + H2O
31% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
guanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-IMP + H2O
28% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
inosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-UMP + H2O
22% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
uridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
ATP + H2O
40% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
?
-
-
-
?
3.1.3.31
p-nitrophenyl phosphate + H2O
140% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
p-nitrophenol + phosphate
-
81066
Rattus norvegicus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.31
additional information
-
serum albumin or dithiothreitol stabilizes cytosolic enzyme at 37°C
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.31
4
5
cytosolic enzyme, absence of Mg2+, substrates 3'-AMP and 3'-dAMP
Rattus norvegicus
3.1.3.31
5.5
-
cytosolic enzyme, presence of Mg2+, substrate 3'-dCMP
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.31
KF
cytosolic enzyme
Rattus norvegicus
3.1.3.31
Tartrate
cytosolic enzyme
Rattus norvegicus
3.1.3.31
Zn2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.31
0.7
-
3'-AMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
0.9
-
5'-AMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
1.7
-
3'-dAMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
1.7
-
3'-dGMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
1.7
-
3'-GMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
2.6
-
3'-dTMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
2.6
-
5'-dAMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
3.2
-
5'-dTMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
3.7
-
5'-CMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
4
-
p-nitrophenyl phosphate
cytosolic enzyme
Rattus norvegicus
3.1.3.31
4.7
-
5'-GMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
6.2
-
5'-dCMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
8
-
3'-dUMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
12
-
5'-dGMP
cytosolic enzyme
Rattus norvegicus
3.1.3.31
25
-
Glycerol 2-phosphate
cytosolic enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.31
cytosol
-
Rattus norvegicus
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.31
125000
-
cytosolic enzyme, gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.31
spleen cytosol
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.31
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.31
0.256
-
cytosolic enzyme
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.3.31
-20°C, 50 mM Tris-HCl buffer, pH 7.5, 1 mM dithiothreitol, 2 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.31
2',3'-cyclic-AMP + H2O
5% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
2'-AMP + H2O
68% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
2-glycerophosphate + H2O
cytosolic enzyme
81066
Rattus norvegicus
glycerol + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-AMP + H2O
-
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-CMP + H2O
-
81066
Rattus norvegicus
cytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
131% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dAMP + H2O
i.e. 2'-deoxyadenosine 3'-phosphate
81066
Rattus norvegicus
3'-deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
101% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxycytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dCMP + H2O
i.e. 2'-deoxycytidine 3'-phosphate
81066
Rattus norvegicus
deoxycytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
77% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dTMP + H2O
i.e. 2'-deoxythymidine 3'-phosphate
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-dUMP + H2O
-
81066
Rattus norvegicus
deoxyuridine + phosphate
-
-
-
-
3.1.3.31
3'-GMP + H2O
49% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
guanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
3'-UMP + H2O
14% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
uridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-AMP + H2O
49% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
adenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-CMP + H2O
97% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
cytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dAMP + H2O
128% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dAMP + H2O
i.e. 2'-deoxyadenosine 5'-phosphate
81066
Rattus norvegicus
deoxyadenosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dCMP + H2O
i.e. 2'-deoxycytidine 5'-phosphate
81066
Rattus norvegicus
deoxycytidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dGMP + H2O
142% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyguanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dGMP + H2O
i.e. 2'-deoxyguanosine 5'-phosphate
81066
Rattus norvegicus
deoxyguanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dIMP + H2O
46% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyinosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dIMP + H2O
i.e. 2'-deoxyinosine 5'-phosphate
81066
Rattus norvegicus
deoxyinosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
60% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dTMP + H2O
i.e. 2'-deoxythymidine 5'-phosphate
81066
Rattus norvegicus
deoxythymidine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dUMP + H2O
28% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
deoxyuridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-dUMP + H2O
i.e. 2'-deoxyuridine 5'-phosphate
81066
Rattus norvegicus
deoxyuridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-GMP + H2O
31% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
guanosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-IMP + H2O
28% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
inosine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
5'-UMP + H2O
22% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
uridine + phosphate
-
81066
Rattus norvegicus
?
3.1.3.31
ATP + H2O
40% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
?
-
-
-
?
3.1.3.31
p-nitrophenyl phosphate + H2O
140% of the activity of 3'-AMP, cytosolic enzyme
81066
Rattus norvegicus
p-nitrophenol + phosphate
-
81066
Rattus norvegicus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.31
additional information
-
serum albumin or dithiothreitol stabilizes cytosolic enzyme at 37°C
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.31
4
5
cytosolic enzyme, absence of Mg2+, substrates 3'-AMP and 3'-dAMP
Rattus norvegicus
3.1.3.31
5.5
-
cytosolic enzyme, presence of Mg2+, substrate 3'-dCMP
Rattus norvegicus