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Literature summary extracted from
- Acyl-(acyl-carrier protein) hydrolase from squash cotyledons specific to long-chain fatty acids: purification and characterization (1992), Plant Mol. Biol., 20, 199-206.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 35000 | - |
2 * 35000, SDS-PAGE | Cucurbita moschata |
| 3.1.2.14 | 51000 | - |
isozymes AH1, AH2, TSKgel G3000SW gel filtration | Cucurbita moschata |
| 3.1.2.14 | 57000 | - |
isozyme AH3, TSKgel G3000SW gel filtration | Cucurbita moschata |
| 3.1.2.14 | 59000 | - |
isozyme AH1, gel filtration | Cucurbita moschata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | Cucurbita moschata | - |
isozymes AH1, AH2, AH3 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.2.14 | isozyme AH1 | Cucurbita moschata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.2.14 | cotyledon | - |
Cucurbita moschata | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 11 | - |
isozyme AH1 | Cucurbita moschata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.2.14 | oleoyl-[acyl-carrier protein] + H2O | - |
Cucurbita moschata | oleic acid + acyl-carrier protein | - |
? |  |
| 3.1.2.14 | palmitoyl-[acyl-carrier protein] + H2O | 10% of the activity with oleoyl-[acyl-carrier-protein] | Cucurbita moschata | palmitic acid + acyl-carrier protein | - |
? | |
| 3.1.2.14 | stearoyl-[acyl-carrier protein] + H2O | 10% of the activity with oleoyl-[acyl-carrier-protein] | Cucurbita moschata | stearic acid + acyl-carrier protein | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.2.14 | dimer | 2 * 35000, SDS-PAGE | Cucurbita moschata |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.2.14 | 9.8 | - |
isozymes AH1, AH2 | Cucurbita moschata |
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Release 2023.1 (January 2023)
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