Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Allais, J.J.; Louktibi, A.; Baratti, J.
    Oxidation of methanol by the yeast, Pichia pastoris. Purification and properties of the formaldehyde dehydrogenase (1983), Agric. Biol. Chem., 47, 1509-1516.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 Cd2+ 1 mM, complete inhibition [Candida] boidinii
1.1.1.284 Cd2+ 1 mM, complete inhibition Komagataella pastoris
1.1.1.284 citrate 1 mM, 24% inhibition Komagataella pastoris
1.1.1.284 CN-
-
 [Candida] boidinii
1.1.1.284 CN- 1 mM, 48% inhibition Komagataella pastoris
1.1.1.284 Cu2+
-
 [Candida] boidinii
1.1.1.284 Cu2+ 1 mM, 76% inhibition Komagataella pastoris
1.1.1.284 EDTA 1 mM, 12% inhibition Komagataella pastoris
1.1.1.284 Hg2+
-
 [Candida] boidinii
1.1.1.284 Hg2+ 1 mM, 90% inhibition Komagataella pastoris
1.1.1.284 NADH
-
 [Candida] boidinii
1.1.1.284 NADH product inhibition Komagataella pastoris
1.1.1.284 NO2- 1 mM, 14% inhibition Komagataella pastoris
1.1.1.284 Zn2+
-
 [Candida] boidinii
1.1.1.284 Zn2+ 1 mM, 28% inhibition Komagataella pastoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.24
-
 NAD+
-
 Komagataella pastoris
1.1.1.284 0.43
-
 formaldehyde
-
 Komagataella pastoris
1.1.1.284 0.48
-
 glutathione
-
 Komagataella pastoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 39000
-
 2 * 39000, SDS-PAGE Komagataella pastoris
1.1.1.284 84000
-
 gel filtration Komagataella pastoris

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Kloeckera sp.
-
-
-
1.1.1.284 Komagataella pastoris
-
-
-
1.1.1.284 [Candida] boidinii
-
-
-
3.1.2.12 Komagataella pastoris
-
 IFP 206
-
3.1.2.12 Komagataella pastoris IFP 206
-
 IFP 206
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 Komagataella pastoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 10.2
-
-
 Komagataella pastoris

Storage Stability

EC Number Storage Stability Organism
1.1.1.284 4°C, in presence of glycerol, 70-80% recovery of activity after 7 days Komagataella pastoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Komagataella pastoris S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 [Candida] boidinii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Kloeckera sp. S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Komagataella pastoris IFP 206 S-formylglutathione + NADH + H+
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Komagataella pastoris glutathione + formate
-
 ?
3.1.2.12 S-formylglutathione + H2O
-
 Komagataella pastoris IFP 206 glutathione + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer
-
 [Candida] boidinii
1.1.1.284 dimer 2 * 39000, SDS-PAGE Komagataella pastoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.284 30
-
-
 Kloeckera sp.
1.1.1.284 35
-
-
 [Candida] boidinii
1.1.1.284 47
-
-
 Komagataella pastoris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.284 20 65 20°C: about 60% of maximal activity, 65°C: about 40% of maximal activity Komagataella pastoris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.284 35
-
 10 min, 50% loss of activity Kloeckera sp.
1.1.1.284 52
-
 10 min, 50% loss of activity Komagataella pastoris
1.1.1.284 52
-
 10 min, 50% loss of activity [Candida] boidinii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 0.233
-
 glutathione
-
 Komagataella pastoris
1.1.1.284 0.267
-
 formaldehyde
-
 Komagataella pastoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 7.9
-
-
 Komagataella pastoris
1.1.1.284 8
-
-
 Komagataella pastoris
1.1.1.284 8
-
-
 [Candida] boidinii
1.1.1.284 8
-
-
 Kloeckera sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.284 6.4 9.3 50% of maximal activity at pH 6.4 and at pH 9.3 Komagataella pastoris

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.284 6.1
-
 37°C, 10 min, 50% loss of activity Komagataella pastoris
1.1.1.284 7
-
 37°C, 10 min, about 20% loss of activity Komagataella pastoris
1.1.1.284 8.5
-
 37°C, 10 min, about 30% loss of activity Komagataella pastoris
1.1.1.284 9.4
-
 37°C, 10 min, about 50% loss of activity Komagataella pastoris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Komagataella pastoris
1.1.1.284 NAD+
-
 [Candida] boidinii
1.1.1.284 NAD+
-
 Kloeckera sp.