BRENDA - Enzyme Database show

Purification and properties of S-formylglutathione hydrolase from human liver

Uotila, L.; Koivusalo, M.; J. Biol. Chem. 249, 7664-7672 (1974)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.1.2.12
stabilization by 2-mercaptoethanol or dithiothreitol
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
2,4,6-Trinitrobenzene sulfonate
-
Homo sapiens
3.1.2.12
5,5'-dithiobis(2-nitrobenzoate)
-
Homo sapiens
3.1.2.12
arsenite
-
Homo sapiens
3.1.2.12
ascorbate
-
Homo sapiens
3.1.2.12
CaCl2
-
Homo sapiens
3.1.2.12
Co2+
-
Homo sapiens
3.1.2.12
CuSO4
-
Homo sapiens
3.1.2.12
F-
-
Homo sapiens
3.1.2.12
folate
-
Homo sapiens
3.1.2.12
glutathione
-
Homo sapiens
3.1.2.12
HgCl2
-
Homo sapiens
3.1.2.12
iodoacetate
-
Homo sapiens
3.1.2.12
p-hydroxymercuribenzoate
-
Homo sapiens
3.1.2.12
Zn2+
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.12
-
S-acetylglutathione
-
Homo sapiens
3.1.2.12
0.29
-
S-formylglutathione
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.12
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
30000
-
2 * 30000, SDS-PAGE
Homo sapiens
3.1.2.12
52000
-
gel filtration
Homo sapiens
3.1.2.12
58200
-
calculation from sedimentation and diffusion coefficients
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.2.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.2.12
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.12
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.2.12
4050
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
3.1.2.12
-70C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
S-acetylglutathione + H2O
slight activity
80982
Homo sapiens
glutathione + acetate
-
80982
Homo sapiens
ir
3.1.2.12
S-formylglutathione + H2O
-
80982
Homo sapiens
glutathione + formate
-
80982
Homo sapiens
ir
Subunits
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.2.12
additional information
-
activation energy 4.5 cal/mol
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
6.9
7.1
-
Homo sapiens
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.2.12
4.6
8.6
stable for 30 min at 20C
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
3.1.2.12
stabilization by 2-mercaptoethanol or dithiothreitol
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.2.12
2,4,6-Trinitrobenzene sulfonate
-
Homo sapiens
3.1.2.12
5,5'-dithiobis(2-nitrobenzoate)
-
Homo sapiens
3.1.2.12
arsenite
-
Homo sapiens
3.1.2.12
ascorbate
-
Homo sapiens
3.1.2.12
CaCl2
-
Homo sapiens
3.1.2.12
Co2+
-
Homo sapiens
3.1.2.12
CuSO4
-
Homo sapiens
3.1.2.12
F-
-
Homo sapiens
3.1.2.12
folate
-
Homo sapiens
3.1.2.12
glutathione
-
Homo sapiens
3.1.2.12
HgCl2
-
Homo sapiens
3.1.2.12
iodoacetate
-
Homo sapiens
3.1.2.12
p-hydroxymercuribenzoate
-
Homo sapiens
3.1.2.12
Zn2+
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.2.12
0.12
-
S-acetylglutathione
-
Homo sapiens
3.1.2.12
0.29
-
S-formylglutathione
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.2.12
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.2.12
30000
-
2 * 30000, SDS-PAGE
Homo sapiens
3.1.2.12
52000
-
gel filtration
Homo sapiens
3.1.2.12
58200
-
calculation from sedimentation and diffusion coefficients
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.2.12
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.2.12
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3.1.2.12
4050
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.2.12
-70C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.2.12
S-acetylglutathione + H2O
slight activity
80982
Homo sapiens
glutathione + acetate
-
80982
Homo sapiens
ir
3.1.2.12
S-formylglutathione + H2O
-
80982
Homo sapiens
glutathione + formate
-
80982
Homo sapiens
ir
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.2.12
dimer
2 * 30000, SDS-PAGE
Homo sapiens
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.2.12
additional information
-
activation energy 4.5 cal/mol
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.2.12
6.9
7.1
-
Homo sapiens
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.1.2.12
4.6
8.6
stable for 30 min at 20C
Homo sapiens