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Literature summary extracted from

  • Takahashi, T.; Yamazaki, Y.; Kato, K.
    Substrate specificity of an alpha-amino acid ester hydrolase produced by Acetobacter turbidans A.T.C.C. 9325 (1974), Biochem. J., 137, 497-503.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.43 ampicillin hydrolysis, substrate inhibition Acetobacter pasteurianus
3.1.1.43 ethyl D-alpha-aminophenylacetate hydrolysis, substrate inhibition Acetobacter pasteurianus
3.1.1.43 methyl D-alpha-aminophenylacetate hydrolysis, substrate inhibition Acetobacter pasteurianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.43 0.5
-
 S-benzyl-L-cysteine ethyl ester
-
 Acetobacter pasteurianus
3.1.1.43 0.6
-
 ampicillin
-
 Acetobacter pasteurianus
3.1.1.43 0.8
-
 L-valine methyl ester
-
 Acetobacter pasteurianus
3.1.1.43 2.6
-
 ethyl DL-alpha-amino-n-butyrate
-
 Acetobacter pasteurianus
3.1.1.43 4.3
-
 methyl D-alpha-aminophenylacetate
-
 Acetobacter pasteurianus
3.1.1.43 5
-
 glycine ethyl ester
-
 Acetobacter pasteurianus
3.1.1.43 24
-
 methyl L-alpha-aminophenylacetate
-
 Acetobacter pasteurianus
3.1.1.43 32.2
-
 methyl DL-alpha-aminoisobutyrate
-
 Acetobacter pasteurianus
3.1.1.43 40.9
-
 D-alanine methyl ester
-
 Acetobacter pasteurianus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.43 Acetobacter pasteurianus
-
 ATCC 9325
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.43
-
 Acetobacter pasteurianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.43 93.5
-
-
 Acetobacter pasteurianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.43 alpha-aminoisobutyric acid methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
 Acetobacter pasteurianus methanol + ?
-
 ?
3.1.1.43 alpha-aminoisobutyric acid methyl ester + H2O
-
 Acetobacter pasteurianus alpha-aminoisobutyric acid + methanol
-
 ?
3.1.1.43 D-alanine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
 Acetobacter pasteurianus methanol + ?
-
 ?
3.1.1.43 D-alanine methyl ester + H2O
-
 Acetobacter pasteurianus D-alanine + methanol
-
 ?
3.1.1.43 D-alpha-phenylglycine methyl ester + 7-aminocephalosporanic acid
-
 Acetobacter pasteurianus methanol + ?
-
 ?
3.1.1.43 D-alpha-phenylglycine methyl ester + H2O
-
 Acetobacter pasteurianus D-alpha-phenylglycine + methanol
-
 ?
3.1.1.43 D-methionine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
 Acetobacter pasteurianus methanol + ?
-
 ?
3.1.1.43 D-methionine methyl ester + H2O
-
 Acetobacter pasteurianus D-methionine + methanol
-
 ?
3.1.1.43 ethyl DL-alpha-amino-n-butyrate + H2O
-
 Acetobacter pasteurianus ?
-
 ?
3.1.1.43 glycine ethyl ester + 7-aminocephalosporanic acid
-
 Acetobacter pasteurianus ethanol + ?
-
 ?
3.1.1.43 glycine ethyl ester + H2O
-
 Acetobacter pasteurianus glycine + ethanol
-
 ?
3.1.1.43 L-cysteine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
 Acetobacter pasteurianus methanol + ?
-
 ?
3.1.1.43 L-cysteine methyl ester + H2O
-
 Acetobacter pasteurianus L-cysteine + methanol
-
 ?
3.1.1.43 L-serine methyl ester + 7-amino-3-deacetoxycephalosporanic acid
-
 Acetobacter pasteurianus methanol + ?
-
 ?
3.1.1.43 L-serine methyl ester + H2O
-
 Acetobacter pasteurianus L-serine + methanol
-
 ?
3.1.1.43 L-valine methyl ester + H2O
-
 Acetobacter pasteurianus L-valine + methanol
-
 ?
3.1.1.43 S-benzyl-L-cysteine ethyl ester + H2O
-
 Acetobacter pasteurianus S-benzyl-L-cysteine + ethanol
-
 ?