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Literature summary extracted from

  • Ibrik, A.; Chahinian, H.; Rugani, N.; Sarda, L.; Comeau, L.C.
    Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium (1998), Lipids, 33, 377-384.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Triton X-100
-
 Penicillium cyclopium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Penicillium cyclopium
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 28000
-
 1 * 28000, SDS-PAGE Penicillium cyclopium
3.1.1.3 29000
-
 gel filtration Penicillium cyclopium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Penicillium cyclopium
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.3 no modification no carbohydrate detected Penicillium cyclopium

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3
-
 Penicillium cyclopium

Storage Stability

EC Number Storage Stability Organism
3.1.1.3 -20°C, stable for months Penicillium cyclopium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information highly active against all triacylglycerols and very low activity against partial acylglycerols or monoesters Penicillium cyclopium ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Penicillium cyclopium butyric acid + ?
-
 ?
3.1.1.3 trioctanoin + H2O
-
 Penicillium cyclopium octanoic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 28000, SDS-PAGE Penicillium cyclopium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30
-
 pH 8, 1 h, about 10% loss of activity Penicillium cyclopium
3.1.1.3 35
-
 60 min, stable Penicillium cyclopium
3.1.1.3 45
-
 10 min, complete loss of activity Penicillium cyclopium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8 10
-
 Penicillium cyclopium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 6 10 pH 6: about 55% of maximal activity, pH 10.0: about 95% of maximal activity Penicillium cyclopium